• Hermalina Sinay
  • Max H Lesilolo Program Studi Pendidikan Biologi, FKIP Universitas Pattimura
Keywords: stomatal, structure, leaf, pegagan


The purpose of this study was to determine the anatomical structure of pegagan leaves through stomata characterization at several different locations on Ambon Island. Survey method was used in this research. The sample was taken in nine villages were spread from lowlands to the highlands on Ambon Island. The procedure of semi-permanent preparations was done by whole mount method. Stomata characterization was done towards the stomata number, stomata density, and stomata index. The collected data were analyzed by descriptive analysis and performed in the form of table or figure. the research result shows that leaf with high stomata number was tend to high in stomata density and stomata index. Based on this research result, it could be conclude that there are no difference of stomatal structure of Pegagan leaves in all location were sample collected. The difference has shown by numbers, density, and stomatal index. The highest Numbers, density and stomatal index shown on Pegagan leaves in lowland, and the lowest that shown in this research on Pegagan leaves in highland


Download data is not yet available.


Cason, S., & Gray, J. E. (2008). Influence of environmental factors on stomatal development. New Phytologist , (178), 9–23

de Padua, L.S., Bunyapraphatsara, N., & Lemmens, R.H.M.J. (1999). Plant Resources of South East Asia. Backhuys Publishers, Leiden, 12(1).

Dalimartha, S. 2000. Atlas Tumbuhan Obat Indonesia 2. Jakarta: Trubus Agriwidya. 214 hal.

Haworth, M., Heath, J., & McElwain, J.C. (2010). Differences in the response sensitivity of stomatal index to atmospheric CO2 among four genera of Cupressaceae conifers. Annals Botany, 105(3), 411–418. Published online 2010 Jan 20. doi: 10.1093/aob/mcp309

Haryanti, S. (2010). Jumlah dan distribusi stomata pada daun beberapa spesies tanaman dikotil dan monokotil. Buletin Agronomi dan Fisiologi, XVIII (2), 21-28

Hanif, U., Mukhtar, A., Ud Din Khan, Z., Hussain, T., Jabeen, R., & Butt, G. Y. (2016). Anatomical study of two Hydrophytes – Pistia stratiotes L. and Centella asiatica (L.) Urban. BIOLOGIA (PAKISTAN), 62 (1), 151-155

Lestari, E.G. 2006. Hubungan antara kerapatan stomata dengan ketahanan kekeringan pada somaklon Padi Gajahmungkur, Towuti dan IR64. Biodiversitas 7 (1): 44-48

Lenny L.R. Kouwenberg Jennifer C. McElwain. (2007). The effect of light intensity and temperature changes on the stomatal and epidermal morphology of Quercus kelloggi: implications for paleoelevation reconstruction. Department of Geology Field Museum of Natural History 1400 South Lake Shore Drive Chicago, 60605, IL, USA lkouwenberg@fmnh.org

Li, X., Li, Y., Zhang, Z., & Li, X. (2015). Influences of Environmental Factors on Leaf Morphology of Chinese Jujubes. PLoS ONE, 10(5), e0127825. http://doi.org/10.1371/journal.pone.0127825.

Menzah, D.B. (2012). Leaf anatomical variation in relation to stress Tolerance among some woody species on the accra plains of Ghana. Journal of Plant Development,19, 13-22.

Momokawa, N., Kadono, Y., & Kudoh, H. (2011). Effects of light quality on leaf morphogenesis of a heterophyllous amphibious plant, Rotala hippuris. Annals Botany, 108(7), 1299–1306. Published online 2011 Sep 6. doi: 10.1093/aob/mcr236

Poole, I., Lawson, T., Weyers, J.D.B., Raven, J.A. (2000). Effect of elevated CO2 on the stomatal distribution and leaf physiology of Ilnus glutinosa. New Pytologist, 145, 511-521.

Ruzin, S.E. Plant Microtechnique and Microscopy. New York Oxford : Oxford University Press

Sinay, H., Arumingtyas, E.E., Harijati, N., & Indriyani, S. (2015). Stomata characterization of local corn cultivars which is grown under field condition in Kisar Island Southwest Maluku Regency. Proceeding The 5th Annual Basic Science International Conference, 5(1), 96-99.

Sundari, T., & Atmaja, R. P. (2011). Bentuk sel epidermis, tipe dan indeks stomata 5 genotipe kedelai pada tingkat naungan berbeda. Journal Biologi Indonesia, 7(1), 67-79.Buletin Agronomi dan Fisiologi, XVIII (2), 21-28

Taub, D. (2010) Effects of rising atmospheric concentrations of carbon dioxide on plants. Nature Education Knowledge, 3(10), 21

Van Steenis,C.G.G.J. 1992. Flora. Penerjemah : M.Soeryowinoto, dkk. Cetakan 5. PT.Pradnya Paramita.Jakarta.

Wallis, T.E. 1965. Analytical Mycroscopy. Boston Little Brown and Company

Xu-Yang, Y., Xiao-Ying, L., Zhi-Gang, X., & Xue-Lei. J. (2017). Effect of light intensity on leaf microstructure and growth of rape seedlings cultivated under a combination of red and blue LEDs. Journal of Integrative Agriculture, 16(1), 97–105.

Xu, Z., & G. Zhou. (2008). Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass. Journal of Experimental Botany, 59, 12: 3317-3325

How to Cite
Sinay, H., & Lesilolo, M. (2020). KARAKTERISASI STOMATA DAUN PEGAGAN (Centella asiatica (L.) Urban.) PADA KETINGGIAN WILAYAH BERBEDA DI PULAU AMBON. Science Map Journal, 2(1), 1-7. https://doi.org/10.30598/jmsvol2issue1pp1-7