Stability Indicating RP-HPLC for Quantification Mangiferin in Extract of Three Species Mango Leaves

  • Yuni Retnaningtyas Faculty of pharmacy, Jember University
  • Nia Kristiningrum Departement of Chemistry, Faculty of Pharmacy, University of Jember Jl. Kalimantan 37 Kampus Tegal Boto Jember
  • Hidayah Dwi Renggani Departement of Chemistry, Faculty of Pharmacy, University of Jember Jl. Kalimantan 37 Kampus Tegal Boto Jember
  • Indah Purnama Sary Departement of Chemistry, Faculty of Pharmacy, University of Jember Jl. Kalimantan 37 Kampus Tegal Boto Jember
DOI: https://doi.org/10.30598/10.30598//ijcr.2020.8-yun
Keywords: Mangiferin, Mango leaf, Mangifera odorata Griff., Mangifera foetida Lour, Mangifera indica L., RP-HPLC, validation

Abstract

The stability indication of Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) method was validated for quantitative determination of mangiferin on three species mango leaves (Mangifera odorata Griff, Mangifera foetida Lour, and Mangifera indica L.). The samples were extracted by maseration method using methanol and concentrated using rotary evaporator. The method carried out on stationary phase a purospher RP-18 endcapped (25 cm × 4.6 mm i.d., 5 µm) column with a mobile phase consisting of methanol: phosphoric acid 0.1% (v/v) (31:69); flow rate:0.8 mL/min; solvent methanol, detection was carried out at 258 nm. The analytical  performace this measurement is good with the value of linearity (r2=0.998), precision (%RSD=0.649%), and accuration (10.67%). The forced degradation studies were carried out according to the International Conference on Harmonization (ICH) guidelines. The results indicating that the complete separation between degradation products and mangiferin peak occured. The degradation limit of mangiferin 5–20% (according to the guideline of ICH) except in basic condition (100%). The method was succesful applied to determine of the mangiferin in  pakel (Mangifera foetida), kweni (Mangifera indica) and kopyor (Mangifera odorata) extract. The mangiferin content was obtained are pakel (9.95%), kopyor (7.40%) and kweni (Mangifera odorata) (2.49%) respectively.

Downloads

Download data is not yet available.
Published
2020-05-31
How to Cite
(1)
Retnaningtyas, Y.; Kristiningrum, N.; Renggani, H.; Sary, I. Stability Indicating RP-HPLC for Quantification Mangiferin in Extract of Three Species Mango Leaves. Indo. J. Chem. Res. 2020, 8, 15-20.
Issue
Vol 8 No 1 (2020): Edisi Bulan Mei (Edition for May)
Section
Research articles

Copyright (c) 2020 Yuni Retnaningtyas, Nia Kristiningrum, Hidayah Dwi Renggani, Indah Purnama Sary

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Copyright on any article is retained by the author(s).
  2. The author grants the journal, the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.
  3. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  4. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
  5. The article and any associated published material is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.