Sintesis Tetrapeptida Linear (DPAP) Menggunakan Metode Sintesis Peptida Fasa Padat (SPPS) Dan Aktivitas Insektisidanya Terhadap Ulat Krop Kubis

Synthesis of A Linear Tetrapeptide (DPAP) Using Solid Phase Peptide Synthesis (SPPS) Methode and Insecticidal Activity Toward Cabbage Cluster Caterpillar

  • Eka Fitri Yanti Fakultas Keguruan dan Ilmu Pendidikan, Universitas Bondowoso, Jl. Diponegoro No.247 Bondowoso
  • Rani Maharani Departemen Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Padjadjaran Jl. Raya Bandung Sumedang KM.21, Jatinangor
DOI: https://doi.org/10.30598/ijcr.2020.8-eka
Keywords: Tetrapeptide, TMOF, Solid phase peptide synthesis, 2-chlorotrytil chloride, Crocidolomia pavonana

Abstract

Crop cabbage worm (Crocidolomia pavonana) is one of the pests that has a bad effect on the cabbage production in Indonesia. One of the efforts that has been applied to control this pest is by using bayrusil. However, the bayrusil can cause negative effects such as poisoning and environmentally polluting effects. To prevent the undesired effects, it has been developed the use of environmentally friendly insecticide and one of them is the use of insecticidal peptide. Tripsin-modulating oostatic factor (TMOF) and ist analogues is one of the peptides that has an insecticidal activity. The aim of this research is to synthesise a linear tetrapeptide (DPAP) an analogues of TMOF and to test insecticidal activities against crop cabbage worm.  A DPAP has been synthesised by Solid Phase Peptide Synthesis on 2-chlorotrytil chloride resin. Fmoc strategy was applied on the synthesis and a combination DIC/Oxime was employed in the coupling reaction.The tetrapeptidyl resin was cleaved by TFA:water:EDT (90:5:5). The peptide was purified by reverse-phase column chromatography and characterized by TOF ES-MS spectroscopy. A linear peptide was biologically tested towards the C. pavonana, showing percentage of mortality values at 1000 ppm.

Downloads

Download data is not yet available.
Published
2020-05-31
How to Cite
(1)
Yanti, E.; Maharani, R. Sintesis Tetrapeptida Linear (DPAP) Menggunakan Metode Sintesis Peptida Fasa Padat (SPPS) Dan Aktivitas Insektisidanya Terhadap Ulat Krop Kubis. Indo. J. Chem. Res. 2020, 8, 6-14.
Issue
Vol 8 No 1 (2020): Edisi Bulan Mei (Edition for May)
Section
Research articles

Copyright (c) 2020 Eka Fitri Yanti, Rani Maharani

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Copyright on any article is retained by the author(s).
  2. The author grants the journal, the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.
  3. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  4. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
  5. The article and any associated published material is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.