Isolasi, Identifikasi, Karakterisasi Dan Uji Toksisitas Senyawa Metabolit Sekunder Fraksi Nonpolar Akar Babandotan (Ageratum conyzoides L)

Isolation, Identification, Characteriterization, And Toxicity Essay Of Nonpolar Secondary Metabolite Fraction From Ageratum conyzoides L

  • Muhammad Irmawan Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl.Perintis Kemerdekaan km 10, Makassar-90245
  • Frederyk Mandey Department of Chemistry, Faculty Mathematic and Natural Science, Hasanuddin University
  • Seniwati Dali Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl.Perintis Kemerdekaan km 10, Makassar-90245
Keywords: Root of Ageratum Conyzoides L., Brine Shrimp Lethality Test (BSLT), isolation, stigmasterol

Abstract

Ageratum Conyzoides L. has a toxic and bioactive component as anti-cancer and especially for the root, it has anti-tumor. In order to figure out the active component, it is necessary to do four steps; isolation, identification, characterization, and toxicity essay Brine Shrimp Lethality Test (BSLT) of non-polar fraction Ageratum conyzoides L plant. Isolation method consist of four parts; extraction, partitioning of Kupchan method, fractionation, and purification. Fractionation method uses press column chromatography (KTT). Furthermore, purification process uses thin layer chromatography (TLC) and characterization with spectroscopy FTIR, 1H-NMR and 13C-NMR. Then characterization utilizes spectroscopy FTIR, 1H-NMR and 13C-NMR. The toxicity essay toward Artemia Salina Leach n-hexane fraction and dichloromethane fraction showed that the fraction had toxic properties with number of LC50 are 0,0237 and 4,642 µg/mL respectively. Moreover, n-hexane fraction  has succeed in isolating Stigmasterol compound shaped white crystalline needles with 19.6 mg weight, 143-144 °C melting point and and had toxic properties with number of LC50 are 30,33 µg/mL.

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Published
2018-07-31
How to Cite
(1)
Irmawan, M.; Mandey, F.; Dali, S. Isolasi, Identifikasi, Karakterisasi Dan Uji Toksisitas Senyawa Metabolit Sekunder Fraksi Nonpolar Akar Babandotan (Ageratum Conyzoides L). Indo. J. Chem. Res. 2018, 6, 1-5.