Isolasi, Identifikasi, Karakterisasi Dan Uji Toksisitas Senyawa Metabolit Sekunder Fraksi Nonpolar Akar Babandotan (Ageratum conyzoides L)

Isolation, Identification, Characteriterization, And Toxicity Essay Of Nonpolar Secondary Metabolite Fraction From Ageratum conyzoides L

  • Muhammad Irmawan Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl.Perintis Kemerdekaan km 10, Makassar-90245
  • Frederyk Mandey Department of Chemistry, Faculty Mathematic and Natural Science, Hasanuddin University
  • Seniwati Dali Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl.Perintis Kemerdekaan km 10, Makassar-90245
DOI: https://doi.org/10.30598//ijcr.2018.6-muh
Keywords: Root of Ageratum Conyzoides L., Brine Shrimp Lethality Test (BSLT), isolation, stigmasterol

Abstract

Ageratum Conyzoides L. has a toxic and bioactive component as anti-cancer and especially for the root, it has anti-tumor. In order to figure out the active component, it is necessary to do four steps; isolation, identification, characterization, and toxicity essay Brine Shrimp Lethality Test (BSLT) of non-polar fraction Ageratum conyzoides L plant. Isolation method consist of four parts; extraction, partitioning of Kupchan method, fractionation, and purification. Fractionation method uses press column chromatography (KTT). Furthermore, purification process uses thin layer chromatography (TLC) and characterization with spectroscopy FTIR, 1H-NMR and 13C-NMR. Then characterization utilizes spectroscopy FTIR, 1H-NMR and 13C-NMR. The toxicity essay toward Artemia Salina Leach n-hexane fraction and dichloromethane fraction showed that the fraction had toxic properties with number of LC50 are 0,0237 and 4,642 µg/mL respectively. Moreover, n-hexane fraction  has succeed in isolating Stigmasterol compound shaped white crystalline needles with 19.6 mg weight, 143-144 °C melting point and and had toxic properties with number of LC50 are 30,33 µg/mL.

Downloads

Download data is not yet available.
Published
2018-07-31
How to Cite
(1)
Irmawan, M.; Mandey, F.; Dali, S. Isolasi, Identifikasi, Karakterisasi Dan Uji Toksisitas Senyawa Metabolit Sekunder Fraksi Nonpolar Akar Babandotan (Ageratum Conyzoides L). Indo. J. Chem. Res. 2018, 6, 1-5.
Issue
Vol 6 No 1 (2018): Edisi Bulan Juli (Edition For July)
Section
Research articles

Copyright (c) 2018 Muhammad Irmawan, Frederyk Mandey, Seniwati Dali

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Copyright on any article is retained by the author(s).
  2. The author grants the journal, the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.
  3. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  4. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
  5. The article and any associated published material is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.