Phytochemical Screening and TLC Profiling of Combination Extracts of Avocado (Persea americana Mill.) and Papaya (Carica papaya) Leaves from Timor Island

  • Marianus Kafelau Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Jl. San Juan, East Nusa Tenggara, Indonesia
  • Aloisius Masan Kopon Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Jl. San Juan, East Nusa Tenggara, Indonesia
  • Anselmus Boy Baunsele Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Jl. San Juan, East Nusa Tenggara, Indonesia
  • Maria Benediktas Tukan Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Jl. San Juan, East Nusa Tenggara, Indonesia
  • Maria Uron Leba Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Jl. San Juan, East Nusa Tenggara, Indonesia
  • Faderina Komisia Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Jl. San Juan, East Nusa Tenggara, Indonesia
  • Erly Grizca Boelan Department of Chemistry Education, Faculty of Teacher Training and Education, Universitas Katolik Widya Mandira, Jl. San Juan, East Nusa Tenggara, Indonesia
DOI: https://doi.org/10.30598/ijcr.2022.10-boe
Keywords: Phytochemical Screening, secondary metabolite compounds, combination extracts, Thin Layer Chromatography

Abstract

Traditionally in the village of Alor, East Nusa Tenggara Province, a combination of avocado and papaya leaves is often used to treat malaria. To be able to be developed as an ingredient in traditional medicine, it is necessary to conduct preliminary research to determine the content of secondary metabolites contained in the combination of avocado and papaya leaves. This study aims to determine the secondary metabolites contained in avocado and papaya leaves through phytochemical screening and Thin Layer Chromatography (TLC) analysis. Extraction was carried out using methanol solvent by maceration, and the extract yield was 91.4%. Testing the content of secondary metabolites in avocado and papaya leaf extracts was carried out by phytochemical screening. Furthermore, the TLC test was carried out to confirm the presence of a positive group of compounds on phytochemical screening, and to determine the chromatographic profile of the extract. After being tested by phytochemical and TLC analysis, the extract contains several metabolites, including flavonoids, alkaloids, tannins, saponins, and steroids. The TLC system used in this study can separate the phytochemical content and give positive results that confirm the results of the phytochemical screening.

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Published
2022-05-30
How to Cite
(1)
Kafelau, M.; Kopon, A.; Baunsele, A.; Tukan, M.; Leba, M.; Komisia, F.; Boelan, E. Phytochemical Screening and TLC Profiling of Combination Extracts of Avocado (Persea Americana Mill.) and Papaya (Carica Papaya) Leaves from Timor Island. Indo. J. Chem. Res. 2022, 10, 32-37.
Issue
Vol 10 No 1 (2022): Edition for May 2022
Section
Research articles

Copyright (c) 2022 Marianus M. Kafelau, Aloisius Masan Kopon, Anselmus Boy Baunsele, Maria Benediktas Tukan, Maria Uron Leba, Faderina Komisia, Erly Grizca Boelan

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