Molecular Networking to Screen Macroalgal Secondary Metabolites: Case for West Timor Macroalgae

  • Welem Turupadang Faculty of Maritime Animal Husbandry and Fisheries, Universitas Nusa Cendana, Jalan Adi Sucipto Penfui 85001 Kupang, Nusa Tenggara Timur
  • Marskel Johanis Office of Maritime Affairs and Fisheries of Kupang Regency, Nusa Tenggara Timur
DOI: https://doi.org/10.30598//ijcr.2023.10-tur
Keywords: GNPS, Macroalgae, 1H NMR, Molecular networking, Spectra

Abstract

The research describes how molecular networking was used as a screening tool to prioritize the isolation workflow of 40 macroalgae sampled from West Timor waters, Indonesia, in addition to a Nuclear Magnetic Resonance-based (NMR) spectroscopy strategy. A mass spectrometry (MS) was employed to generate spectra that later be used as data to produce the molecular network with the Global Natural Product Social Molecular Networking (GNPS) website. The screening process used the molecular network, which assisted in the selection of six samples of macroalgae out of the 40 samples for further examination. Lastly, an NMR-based protocol was employed to choose the samples of interest to be investigated further. Six samples were shortlisted from the GNPS molecular network based on specimens, which were then validated with 1H NMR spectroscopy to finally prioritized three samples.

Downloads

Download data is not yet available.
Published
2023-01-31
How to Cite
(1)
Turupadang, W.; Johanis, M. Molecular Networking to Screen Macroalgal Secondary Metabolites: Case for West Timor Macroalgae. Indo. J. Chem. Res. 2023, 10, 203-211.
Issue
Vol 10 No 3 (2023): Edition for January 2023
Section
Research articles

Copyright (c) 2023 Welem Turupadang, Marskel Johanis

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Copyright on any article is retained by the author(s).
  2. The author grants the journal, the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.
  3. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  4. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
  5. The article and any associated published material is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.