Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)

Antioxidant Activity Test Of Methanol Extract Of Kesumba Keling (Bixa orellana L) Seeds

  • Fensia Analda Souhoka Department of Chemistry, Faculty Mathematic and Natural Science, Pattimura University, Jl. Ir. Putuhena No. 1 Poka Ambon 97233-Maluku
  • Nikmans Hattu Department of Chemistry, Faculty of Matemathic and Natural Sciences, Pattimura University, Ambon 97233
  • Marsye Huliselan Department of Chemistry, Faculty of Matemathic and Natural Sciences, Pattimura University, Ambon 97233
DOI: https://doi.org/10.30598/ijcr.2019.7-fas
Keywords: Antioxidant activity, kesumba keling seeds, methanol extract, phytochemical test.

Abstract

Kesumba keling (Bixa orellana L) has been widely used as a natural dye on lips, hair, and cloth. The red pigment in kesumba keling seeds comes from a bixin and norbixin compound which have many conjugated double bonds, so it has the potential of antioxidants. This study aims to determine the antioxidant compound and an antioxidant activity of methanol extract of kesumba keling seeds. The moisture content of kesumba keling seeds is 78.74%. The powder of kesumba keling seeds was extracted using maceration method with 80% of methanol. Phytochemical test results of methanol extract positively contained flavonoid compound. An antioxidant activity test of methanol extract of kesumba keling seeds was carried out by determining DPPH free radical deterrent activity. The absorbance measurement were made using a UV-Vis spectrophotometer at a wavelength of 517 nm. Kesumba keling seeds extract has antioxidant activity which is indicated by IC50 value of 69.425 ppm, so it is classified as a strong antioxidant.

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Published
2019-07-31
How to Cite
(1)
Souhoka, F.; Hattu, N.; Huliselan, M. Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa Orellana L). Indo. J. Chem. Res. 2019, 7, 25-31.
Issue
Vol 7 No 1 (2019): Edisi Bulan Juli (Edition For July)
Section
Research articles

Copyright (c) 2019 Fensia Analda Souhoka, Nikmans Hattu, Marsye Huliselan

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