IMBANGAN CPO DAN MINYAK LIMBAH IKAN PATIN DISUPLEMENTASI EMULSIFIER LYSOFORTE DALAM RANSUM TERHADAP AKTIVITAS ENZIM PENCERNAAN BROILER
CPO AND CATFISH WASTE OIL BALANCE SUPPLEMENTED WITH LYSOFORTE EMULSIFIER IN RATIONS ON DIGESTIVE ENZYME ACTIVITY OF BROILER CHICKENS
Abstract
Crude Palm Oil (CPO) dan minyak limbah ikan patin mengandung lemak sehingga dapat dimanfaatkan sebagai sumber energi dalam ransum broiler. Penambahan emulsifier dimaksudkan untuk meningkatkan penyerapan lemak. Penelitian ini bertujuan untuk mengetahui pengaruh imbangan CPO dan minyak limbah ikan patin yang disuplementasi emulsifier lysoforte dalam ransum terhadap aktivitas enzim pada duodenum broiler. Percobaan dilakukan terhadap 120 ekor sampel broiler strain Cobb, menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan dan empat ulangan. Perlakuannya meliputi P1 (ransum mengandung 3% CPO+emulsifier lysoforte), P2 (ransum mengandung 2% CPO+1% MLIP+emulsifier lysoforte), P3 (ransum mengandung 1,5% CPO+1,5% MLIP+emulsifier lysoforte), P4 (ransum mengandung 1% CPO+2% MLIP+emulsifier lysoforte), dan P5 (ransum mengandung 3% MLIP+emulsifier lysoforte). Variabel yang diamati meliputi aktivitas enzim amilase, protease, dan lipase. Hasil penelitian menunjukkan penambahan minyak limbah ikan patin dan CPO yang disuplementasi dengan emulsifier lysoforte memberikan hasil yang berbeda nyata terhadap aktivitas enzim amilase dan lipase, namun tidak berbeda nyata terhadap aktivitas enzim protease. Ransum broiler P3 yang mengandung 1,5% CPO, 1,5% minyak limbah ikan patin dan emulsifier lysoforte memberikan hasil yang paling baik terhadap aktivitas enzim amilase dan lipase dibanding perlakuan lainnya.
ABSTRACT
Crude Palm Oil (CPO) and catfish waste oil contain fat, making them suitable energy sources in broiler rations. The addition of emulsifiers is intended to increase fat absorption. This study aimed to determine the effect of the balance of CPO and catfish waste oil supplemented with Lysoforte emulsifier in the ration on enzyme activity in the broiler duodenum. The experiment was conducted on 120 Cobb strain broiler samples, using a Completely Randomized Design (CRD) with five treatments and four replications. The treatments included P1 (ration containing 3% CPO+lysoforte emulsifier), P2 (ration containing 2% CPO+1% MLIP+lysoforte emulsifier), P3 (ration containing 1.5% CPO+1.5% MLIP+lysoforte emulsifier), P4 (ration containing 1% CPO+2% MLIP+lysoforte emulsifier), and P5 (ration containing 3% MLIP+lysoforte emulsifier). The observed variables included amylase, protease, and lipase enzyme activity. The results showed that the addition of catfish waste oil and CPO supplemented with lysoforte emulsifier gave significantly different results on amylase and lipase enzyme activity, but not significantly different on protease enzyme activity. The P3 broiler ration containing 1.5% CPO, 1.5% catfish waste oil, and lysoforte emulsifier gave the best results on amylase and lipase enzyme activity compared to other treatments.
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References
Abdollahi, M. R., Wiltafsky-Martin, M., & Ravindran, V. (2021). Application of apparent metabolizable energy versus nitrogen-corrected apparent metabolizable energy in poultry feed formulations: a continuing conundrum. Animals, 11(8), 2174. https://doi.org/10.3390/ani11082174
Abun, A., Haetami, K., Rusmana, D., & Ramdhan, R.F. (2025). Macroscopic physical properties and nutrient content in the fermentation of Pangasius waste by single cultures and microbial consortiums and their potential for feed. Microbiologyopen, 14(4), e70040. https://doi.org/10.1002/mbo3.70040
Ahiwe, E. U., Omede, A. A., Abdallh, M. B., & Iji, P. A. (2018). Managing dietary energy intake by broiler chickens to reduce production costs and improve product quality. Animal husbandry and nutrition, 115, 115-145. https://dx.doi.org/10.5772/intechopen.76972
Alagawany, M., Elnesr, S. S., & Farag, M. R. (2018). The role of exogenous enzymes in promoting growth and improving nutrient digestibility in poultry. Iranian journal of veterinary research, 19(3), 157. https://pubmed.ncbi.nlm.nih.gov/30349560/
Arshad, M.A., Ul-Hassan, F., Bhatti, S.A., Rehman, M.S., Yousaf, W., Younus, G., Sizmaz, o., & Bilal, M.Q. (2021). Supplementation of bile acids and lipase in broiler diets for better nutrient utilization and performance: Potential effects and future implications – A review. Annual Animal Science, 21(3), 757–787. https://doi.org/10.2478/aoas-2020-0099
Aryani, A., Suharman, I., Heltonika, B., Edison, Yulindra, A., & Stefani, N. (2024). Asian redtail catfish (Hemibagrus nemurus) larvae response to commercial feed enriched with fish oil derived from Pangasius waste. BIO Web of Conferences, 136, 01006. https://doi.org/10.1051/bioconf/202413601006
Attia, Y. A., Al-Harthi, M. A., & Abo El-Maaty, H. M. (2020). The effects of different oil sources on performance, digestive enzymes, carcass traits, biochemical, immunological, antioxidant, and morphometric responses of broiler chicks. Frontiers in Veterinary Science, 7, 181. https://doi.org/10.3389/fvets.2020.00181
Ayu, D. F., Diharmi, A., & Ali, A. (2019). Characterization of the oil from the abdomen part of smoked catfish (Pangasius hypophthalmus) processing by-product. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(1), 187-197. https://doi.org/10.17844/jphpi.v22i1.26473
Benzertiha, A., Kierończyk, B., Rawski, M., Kołodziejski, P., Bryszak, M., & Józefiak, D. (2019). Insect oil as an alternative to palm oil and poultry fat in broiler chicken nutrition. Animals, 9(3), 116. https://doi.org/10.3390/ani9030116
[BSN] Badan Standar Nasional. 2015. SNI 8173.2:2015 Tentang Pakan Ayam Ras Pedaging (Broiler) ─ Bagian 2: Masa Awal (Starter) dan SNI 8173.3:2015 Tentang Pakan ayam ras Pedaging (Broiler) ─ Bagian 3: Masa Akhir (Finisher). Jakarta: Badan Standardisasi Nasional.
Choi, J., Kong, B., Bowker, B. C., Zhuang, H., & Kim, W. K. (2023). Nutritional strategies to improve meat quality and composition in the challenging conditions of broiler production: a review. Animals, 13(8), 1386. https://doi.org/10.3390/ani13081386
Cullere, M., Schiavone, A., Dabbou, S., Gasco, L., & Dalle Zotte, A. (2019). Meat quality and sensory traits of finisher broiler chickens fed with black soldier fly (Hermetia illucens L.) larvae fat as alternative fat source. Animals, 9(4), 140. https://doi.org/10.3390/ani9040140
Ding, X. M., Li, D. D., Li, Z. R., Wang, J. P., Zeng, Q. F., Bai, S. P., ... & Zhang, K. Y. (2016). Effects of dietary crude protein levels and exogenous protease on performance, nutrient digestibility, trypsin activity and intestinal morphology in broilers. Livestock Science, 193, 26-31. https://doi.org/10.1016/j.livsci.2016.09.002
Elbaz, A. M., Zaki, E. F., Salama, A. A., Badri, F. B., & Thabet, H. A. (2023). Assessing different oil sources efficacy in reducing environmental heat-stress effects via improving performance, digestive enzymes, antioxidant status, and meat quality. Scientific Reports, 13(1), 20179. https://doi.org/10.1038/s41598-023-47356-6
Febrianto, R., & Sudarmo. (2020). Fish oil production process from waste catfish (Pangasius pangasius) in Balai Besar Pengujian Penerapan Hasil Perikanan (BBP2HP) East Jakarta. Journal od Marine and Coastal Science, 9(2), 65-69. https://doi.org/10.20473/jmcs.v9i2.20251
Fitriliyani, I. (2018). Aktifitas enzim saluran pencernaan ikan nila (Oreohromis niloticus) dengan pakan mengandung tepung daun lamtoro (Leucaena leucophala) terhidrolisis dan tanpa hidrolisis dengan ekstrak enzim cairan rumen domba. Bioscientiae, 8(2), 16-31. https://doi.org/10.20527/b.v8i2.193
Foltyn, M., Lichovníková, M., Rada, V., & Musilová, A. (2015). Apparent ileal digestibility of protein and amino acids in protein feedstuffs and trypsin activity in the small intestine in broiler chickens. Czech J. Anim. Sci., 60(8), 375-382. https://doi.org/10.17221/8407-CJAS
Gaspersz, V. (2006). Teknik Analisis Dalam Penelitian Percobaan. Jilid 1. Cet. ke 3. Bandung: Tarsito. P. 136-140.
Hu, Y. D., Lan, D., Zhu, Y., Pang, H. Z., Mu, X. P., & Hu, X. F. (2017). Effect of diets with different energy and lipase levels on performance, digestibility and carcass trait in broilers. Asian-Australasian journal of animal sciences, 31(8), 1275. https://doi.org/10.5713/ajas.17.0755
Izuddin, W. I., Loh, T. C., Nayan, N., Akit, H., Foo, H. L., & Noor, A. M. (2023). Antioxidant enzyme system modulation by dietary palm oils, palm kernel oil and soybean oil in laying hens. Animals, 13(14), 2245. https://doi.org/10.3390/ani13142245
Jimenez-Moya, B., Barroeta, A.C., Guardiola, F., Soler, M.D., Rodriguez-Sanchez, R., & Sala, R. (2021). Replacement of palm oil with soybean acid oil in broiler chicken diet: fat digestibility and lipid class content along the intestinal tract. Animals (Basel), 11(9), 2586. https://doi.org/10.3390/ani11092586
Kerr, B. J., Pearce, S. C., Risley, C. R., Wilson, B. A., & Koltes, D. A. (2024). Energy digestibility in broilers and poult performance when fed palm or soybean oil with or without glyceryl monolaurate. Poultry Science, 103(12), 104442. https://doi.org/10.1016/j.psj.2024.104442
[KKP] Kementrian Kelautan dan Perikanan. (2024). Kelautan dan Perikanan dalam Angka Tahun 2024. Jakarta: Kementrian Kelautan dan Perikanan.
Lani, R. K., Kasmiyati, S., & Sukmana, A. (2019). Seleksi dan karakterisasi enzim protease dari getah tumbuhan Ficus spp. pada variasi suhu dan pH. In Seminar Nasional Sains & Entrepreneurship, 1(1), 1-5.
Layly, I. R., & Wiguna, N. O. (2016). Studi potensi lipase Alcaligenes faecalis untuk aplikasi biodeterjen. Jurnal Bioteknologi and Biosains Indonesia, 3(2), 66-71. https://scholar.archive.org/fatcat/release/gbczteaiiba27ctveygg2lhbv4
Leeson, S., & Summer, J.D. (2001). Nutrition of The Chicken. Guelph, Ontario, Canada: University Books.
Marliyati, S. A., Rimbawan, & Harianti, R. (2021). Physicochemical And Functional Characteristics Of Red Palm Oil. Jurnal Gizi Masyarakat Indonesia (The Journal of Indonesian Community Nutrition), 10(1), 83-94. https://journal.unhas.ac.id/index.php/mgmi/article/view/20494
Muliasari, H., & Permatasari, L. (2022). Studi awal uji aktivitas enzim amilase dari tumbuhan secara kualitatif berdasarkan perbedaan suhu dan konsentrasi substrat. Journal of Agritechnology and Food Processing, 2(1), 29-34. https://doi.org/10.31764/jafp.v2i1.9338
Musigwa, S., Cozannet, P., Asiamah, C. A., & Wu, S. B. (2024). Effects of dietary protein levels, net energy levels, and essential amino acid-to-true protein ratios on broiler performance. Animals, 14(21), 3065. https://doi.org/10.3390/ani14213065
Nalle, C. L., Toelle, N., & Mawadah, U. (2021). Growth performance and nutrient digestibility of broilers fed different type of diets and feed additives. Journal of the Indonesian Tropical Animal Agriculture, 46(4), 304-324. https://doi.org/10.14710/jitaa.46.4.304-324
Noor, M. L., Diharmi, A., & Karnila, R. (2021). Karakteristik dan profil asam lemak kombinasi minyak ikan patin dan minyak hati ikan hiu. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(1), 122-130. https://doi.org/10.17844/jphpi.v24i1.33821
Oketch, E.O., Lee, J.W., Yu, M., Hong, J.S., Kim, Y.B., Nawarathne, S.R., Chiu, J.W., & Heo J.M. (2022). Physiological responses of broiler chickens fed reduced-energy diets supplemented with emulsifiers. Animal Bioscience, 35(12), 1929-1939. https://doi.org/10.5713/ab.22.0142
Pirgozliev, V., Mansbridge, S. C., Rose, S. P., Mackenzie, A. M., Beccaccia, A., Karadas, F., ... & Bravo, D. (2019). Dietary essential oils improve feed efficiency and hepatic antioxidant content of broiler chickens. Animal, 13(3), 502-508. https://doi.org/10.1017/S1751731118001520
Pratantie, E. M., Bintoro, V. P., & Dwiloka, B. (2021). Isolasi enzim amilase dari kecambah kacang tunggak (Vigna unguiculata). JITEK (Jurnal Ilmiah Teknosains), 7(1), 29-35. https://doi.org/10.26877/jitek.v7i1/Mei.8359
Sa'diyah, H., & Kimia, J. (2019). Isolasi, identifikasi, dan uji aktivitas flavonoid dari buah delima (punica granatum l.) sebagai inhibitor lipase pankreas. Inovasi Kimia Dan Pembelajaran, November, 69–81.
Saminathan, M., Mohamed, W. N. W., Noh, A. M., Ibrahim, N. A., Fuat, M. A., & Ramiah, S. K. (2022). Effects of dietary palm oil on broiler chicken productive performance and carcass characteristics: a comprehensive review. Tropical animal health and production, 54(1), 64. https://doi.org/10.1007/s11250-022-03046-5
Sari, Y. S. I., Suthama, N., & Sukamto, B. (2019). Perkembangan duodenum dan pertambahan bobot badan pada ayam broiler yang diberi ransum dengan protein mikropartikel ditambah probiotik Lactobacillus sp. Jurnal Penelitian Peternakan Terpadu, 1(1), 4-12. https://doi.org/10.36626/jppt.v1i1.148
Shoaib, M., Bhatti, S. A., Ashraf, S., Hamid, M. M. A., Javed, M. M., Amir, S., ... & Saif-ur-Rehman, M. (2023). Fat digestion and metabolism: effect of different fat sources and fat mobilisers in broilers’ diet on growth performance and physiological parameters–a review. Annals of Animal Science, 23(3), 641-661. https://doi.org/10.2478/aoas-2022-0083
Shoaib, M., Bhatti, S. A., Nawaz, H., & Saif-ur-Rehman, M. (2021). Effect of different fat sources and energy levels on growth performance, nutrient digestibility and meat quality in broiler chicks. JAPS: Journal of Animal & Plant Sciences, 31(5). https://doi.org/10.36899/JAPS.2021.5.0325
[SIGL] Saraswati Indo Genetech Laboratorium. (2023). Analisa Summary Report Custumer Laboratory Service. Bogor: Saraswati Indo Genetech Laboratorium
Sizova, E. A., & Ryazantseva, K. V. (2022). Fats and emulsifiers in feeding broiler chickens. Agricultural biology, 57(4), 664-680. https://doi.org/10.15389/agrobiology.2022.4
Tancharoenrat, P., Ravindran, V., Zaefarian, F., & Ravindran, G. (2014). Digestion of fat and fatty acids along the gastrointestinal tract of broiler chickens. Poultry Science, 93(2), 371-379. https://doi.org/10.3382/ps.2013-03344
Taubner, T., Skřivan, M., Englmaierová, M., & Malá, L. (2023). Effects of hemp seed and flaxseed on enzyme activity in the broiler chicken digestive tract. Animal, 17(4), 100765. https://doi.org/10.1016/j.animal.2023.100765
Wan Mohamed, W. N., MD Noh, A., Ibrahim, N. A., & Mookiah, S. (2025). Effects of the inclusion of crude palm oil and palm kernel meal on broiler performance and chicken meat texture. Journal of Central European Agriculture, 26(3), 592-605. https://doi.org/10.5513/JCEA01/26.3.4649
Winurdana, A. S., Sholihah, M. A., & Sofiyana, M. S. (2023). Penggunaan crude palm oil (CPO) berbagai level pada pakan terhadap performa bebek Peking. Journal of Animal Research and Applied Science, 4(2), 71-75. https://doi.org/10.22219/aras.v4i2.31253
Yadav, S., & Jha, R. (2019). Strategies to modulate the intestinal microbiota and their effects on nutrient utilization, performance, and health of poultry. Journal of animal science and biotechnology, 10(1), 2. https://doi.org/10.1186/s40104-018-0310-9
Zavelinski, V.A.B., Vieira, V.I., Bassi, L.S., de Almeida, L.M., Schramm, V.G., Maiorka, A., & de Oliveira, S.G. (2024). The effect of protease supplementation in broiler chicken diets containing maize from different batches on growth performance and nutrient digestibility. Animal, 18(12), 101363. https://doi.org/10.1016/j.animal.2024.101363
