KUALITAS FISIK DAN NUTRIEN JERAMI PADI YANG DIFERMENTASI MIKROORGANISME LOKAL ASAL JERAMI PADI

PHYSICAL AND NUTRIENT QUALITY OF RICE STRAW FERMENTED WITH LOCAL MICROORGANISMS BASED ON RICE STRAW

  • Rizka Ramadhani Program Studi Ilmu Nutrisi dan Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Indonesia
  • Tri Wahyu Apriliana Program Studi Ilmu Nutrisi dan Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Indonesia
  • Dini Andani Putri Program Studi Ilmu Nutrisi dan Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Indonesia
  • Ridha Aini Amanda Program Studi Ilmu Nutrisi dan Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Indonesia
  • Maya Shofiah Program Studi Ilmu Nutrisi dan Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Indonesia
  • Muhammad Farhan Hilmy Adeliza Program Studi Ilmu Nutrisi dan Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Indonesia
  • Edi Kurniawan Program Studi Ilmu Nutrisi dan Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Indonesia
  • Sri Suharti Program Studi Ilmu Nutrisi dan Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Indonesia
  • Dwierra Evvyernie Amirroenas Program Studi Ilmu Nutrisi dan Pakan, Fakultas Peternakan, Institut Pertanian Bogor, Indonesia
Keywords: Fermentation, rice straw, physical quality, nutrient quality, local microorganisms

Abstract

Jerami padi dapat dimanfaatkan secara optimal sebagai sumber pakan alternatif bagi ternak ruminansia melalui fermentasi guna meningkatkan kandungan nutriennya. Penelitian ini bertujuan untuk mengevaluasi lama fermentasi terhadap kualitas fisik dan nutrien jerami padi yang difermentasi menggunakan mikroorganisme lokal (MOL) asal jerami padi. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan variasi lama fermentasi (0, 7, 14, 21, dan 28 hari) dan 4 ulangan. Variabel yang diamati meliputi kualitas MOL, kualitas fisik dan nutrien silase (bahan kering, abu, protein kasar, lemak kasar dan serat kasar). Data yang diperoleh dianalisis dengan Analysis of Variance (ANOVA) dan perbedaan yang nyata antar perlakuan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa MOL jerami padi memiliki karakteristik fisik berwarna coklat tua, aroma khas tape, pH 4, dan kandungan glukosa 6% BRIX. Populasi BAL dan bakteri selulolitik masing-masing berkisar 1,24 × 10⁷ dan 2,89 × 10⁶ CFU/mL. Hasil fermentasi silase amoniasi jerami padi menunjukkan adanya perubahan fisik berupa tekstur yang lebih lunak, aroma menyengat, dan warna coklat. Kandungan protein kasar tertinggi ditunjukkan pada fermentasi 7 hari (12,95%), sedangkan serat kasar terendah ditunjukkan pada fermentasi 21 hari (25,76%). Hasil ini menunjukkan bahwa penggunaan MOL jerami padi berpotensi meningkatkan kualitas silase jerami melalui peningkatan protein dan penurunan serat kasar dimana fermentasi selama 21 hari sebagai durasi optimal untuk meningkatkan kualitas nutrisi jerami padi sebagai pakan ternak ruminansia.

ABSTRACT 

Rice straw can be optimally utilized as an alternative feed source for ruminant livestock through fermentation to increase its nutrient content. This experiment aims to evaluate the effect of fermentation duration on the physical and nutrient quality of rice straw fermented using local microorganisms (MOL) derived from rice straw. A completely randomized design (CRD) was applied with five fermentation periods (0, 7, 14, 21, and 28 days) and four replications. The parameters measured included the quality of LMO, physical characteristics, and silage nutrient composition (dry matter, ash, crude protein, crude fat, and crude fiber). Data were analyzed using Analysis of Variance (ANOVA), followed by Duncan’s test to determine significant differences among treatments. The results showed that rice straw had a dark brown color, distinctive aroma, pH 4, and glucose content of 6% BRIX, with lactic acid bacteria and cellulolytic bacteria populations of 1.24 × 10⁷ and 2.89 × 10⁶ CFU/mL, respectively. Fermented rice straw silage exhibited improved physical quality with a softer texture, pungent aroma, and brownish color. The highest crude protein content was observed after 7 days of fermentation (12.95%), while the lowest crude fiber content was obtained after 21 days (25.76%). These findings indicate that rice straw-based LMO  has the potential to improve the nutritional quality of rice straw silage, with 21 days identified as the optimum fermentation duration for ruminant feed.

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References

Afifah, D.N., Sari, I.R., Prastifani, N.T., Fulyani, F., Anjani, G., Widyastuti, N. and Hastuti, V.N. (2023). Effect of fermentation time on nutrition content, physical properties, pH, amino acids, fatty acids composition and organoleptics on fermented mackerel sausage (Rastrelliger kanagurta Cuvier) characteristics. International Journal of Food Studies, 12(1), 57-70. https://doi.org/10.7455/ijfs/12.1.2023.a4

Aglazziyah, H., Ayuningsih, B., & Khairani, L. (2020). Pengaruh penggunaan dedak fermentasi terhadap kualitas fisik dan pH silase rumput gajah (Pennisetum purpureum). Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan, 2(3), 156-166. https://doi.org/10.24198/jnttip.v2i3.30290

Agustiyani, I., Sari, L. A., Chandra, R., Zahera, R., Permana, I. G. (2021). Comparison between single and mixed-species NIRS databases’ accuracy of dairy fiber feed value detection. IOP Conference Series: Earth and Environmental Science, 667(1), 012103. https://doi.org/10.1088/1755-1315/667/1/012103

Akturk, B., & Gumus, H. (2020). Effect of lactic bacteria inoculant on quality, fermentation profile and nutritive value of alfalfa silage at different ensiling period. Ankara Univ Vet Fak Derg, 67 (3), 281-287. https://doi.org/10.33988/auvfd. 624047

Aman, L., Sio, S., & Bira, G. F. (2022). Pengaruh penggunaan mikroorganisme lokal (MOL) cairan rumen sapi pada level inokulum yang berbeda terhadap nilai kandungan serat jerami padi terfermentasi. Journal of Animal Science (JAS), 7(2), 19-22. https://doi.org/10.32938/ja.v7i2.1676

Amin, M., Hasan, S. D., Yanuarianto, O., & Iqbal, M. (2015). Pengaruh lama fermentasi terhadap kualitas jerami padi amoniasi yang ditambah probiotik Bacillus Sp. Jurnal Ilmu dan Teknologi Peternakan Indonesia, 1(1), 8-13. https://doi.org/10.29303/jitpi.v1i1.4

Aswad, A., & Negara, A. B. W. (2025). Uji kecernaan ransum komplit berbasis silase jerami padi dengan penambahan em4 (effective microorganisme) secara invitro. Journal of Innovation Research and Knowledge, 5(1), 561-568. https://www.bajangjournal.com/index.php/JIRK/article/view/10340

[BPS] Badan Pusat Statistik. (2024). Luas Panen, Produksi, dan Produktivitas Padi Menurut Provinsi. Jakarta: Badan Pusat Statistik.

Brexó, R. P., & Sant’Ana, A. D. S. (2018). Microbial interactions during sugar cane must fermentation for bioethanol production: does quorum sensing play a role?. Critical reviews in biotechnology, 38(2), 231-244. https://doi.org/10.1080/07388551.2017.1332570

Candrasari, D. P., Fitria, R. & Hindratiningrum, N. (2019). Pengaruh perlakuan amoniasi fermentasi (Amofer) terhadap kualitas fisik janggel jagung. Jurnal Ilmiah Ilmu-Ilmu Peternakan, 22(2), 117-123. https://doi.org/10.22437/jiiip.v22i2.8352

Datsomor, O., Yan, Q., Wang, K., Mohamed, S., Opoku-Mensah, L., Zhao, G., & Miao, L. (2022). Effect of ammoniated and/or basidiomycete white-rot fungi treatment on rice straw proximate composition, cell wall component, and in vitro rumen fermentation characteristics. Fermentation, 8(5), 228. https://doi.org/10.3390/fermentation8050228

Dryden, G. M. (2021). Fundamentals of Applied Animal Nutrition. England: ABI Press.

Dwicahya, N., Lifianthi, L., & Wartono, W. (2024). Dampak pembakaran terbuka jerami padi terhadap karakteristik lahan persawahan. Agrienvi: Jurnal Ilmu Pertanian, 18(1), 1-9. https://e-journal.upr.ac.id/index.php/aev/article/view/15251

Falcioni, R., Moriwaki, T., de Oliveira, D.M., Andreotti, G.C., de Souza, L.A., Dos Santos, W.D., Bonato, C.M. and Antunes, W.C. (2018). Increased gibberellins and light levels promotes cell wall thickness and enhance lignin deposition in xylem fibers. Front. Plant Sci., 9, 1391. https://doi.org/10.3389/fpls.2018.01391

Fariani, A., Pratama, A. N. T., & Muslim, G. (2021). The multi-level ammoniation on the digestibility of palm press fiber. Journal of Advanced Veterinary and Animal Research, 8(2), 230. https://doi.org/10.5455/javar.2021.h507

Haryanto, A., Suharyatun, S., Rahmawati, W., & Triyono, S. (2019). Energi terbarukan dari jerami padi: Review potensi dan tantangan bagi Indonesia. Jurnal Keteknikan Pertanian, 7(2), 137-146. https://doi.org/10.19028/jtep.07.2.137-146

Laila, W., Indrayati, S., Zaunit, M. M., & Harleni, H. (2024). Isolasi, karakterisasi, dan uji aktivitas antimikroba bakteri asam laktat dari biji kakao sisa pencernaan musang sebagai kandidat probiotik. Jurnal Kesehatan Perintis, 11(2), 120-127. https://doi.org/10.33653/jkp.v11i2.1102

Li, B., Zhao, C., Sun, Q., Chen, K., Zhao, X., Xu, L., ... & Peng, H. (2023). Effects of ammonification–steam explosion pretreatment on the production of true protein from rice straw during solid-state fermentation. Sustainability, 15(7), 5964. https://doi.org/10.3390/su15075964

Lokapirnasari, W. P., Setiawan, A., & Soetji Prawesthirini, S. U. (2015). Potensi kombinasi bakteri dan jamur selulolitik pada fermentasi bekatul terhadap kandungan serat kasar dan protein kasar. Buletin of Animal Science (Buletin Peternakan), 39(3), 174-179. https://doi.org/10.21059/buletinpeternak.v39i3.7985

Ma, Y., Chen, X., Khan M. Z., Xiao J., & Cao. Z. (2022). A combination of novel microecological agents and molasses role in digestibility and fermentation of rice straw by facilitating the ruminal microbial colonization. Front. Microbiol, 13, 948049. https://doi.org/10.3389/fmicb.2022.948049

Marsiningsih, N. W., Suwastika, A. A. N. G., & Sutari, N. W. S. (2015). Analisis Kualitas Larutan MOL (Mikroorganisme Lokal) Berbasis Ampas Tahu. Jurnal Agroekoteknologi Tropica, 4(3), 180-190. https://ojs.unud.ac.id/index.php/jat/article/view/17285

Mayulu, H., & Suhardi. (2016). Potency and carrying capacity of rice straw for beef cattle feeding in East Kalimantan. Jurnal Ilmu dan Teknologi Peternakan, 4(3), 119-129. https://journal.unhas.ac.id/index.php/peternakan/article/view/1226

Muck, R. E., Nadeau, E. M. G., McAllister, T. A., Contreras-Govea, F. E., Santos, M. C., & Kung Jr, L. (2018). Silage review: Recent advances and future uses of silage additives. Journal of dairy science, 101(5), 3980-4000. https://doi.org/10.3168/jds.2017-13839

Nemet, F., Perić, K., & Lončarić, Z. (2021). Microbiological activities in the composting process: A review. Columella: Journal of Agricultural and Environmental Sciences, 8 (2), 41–53. https://doi.org/10.18380/SZIE.COLUM.2021.8.2.41

Nguyen, G. T. T., Vu, T. M., Wanapat, M., & Nguyen, D. V. (2024). Nutrient digestibility, rumen fermentation and microbial nitrogen synthesis of swamp buffaloes fed urea-lime treated rice straw. Italian Journal of Animal Science, 24(1), 25-32. https://doi.org/10.1080/1828051X.2024.2442034

Nisa, A. K., Lamid, M., Lokapirnasari, W. P., & Amin, M. (2021, February). Improving crude protein and crude fat content of Seligi leaf (Phyllanthus buxifolius) flour through probiotic fermentation. In IOP Conference Series: Earth and Environmental Science (Vol. 679, No. 1, p. 012041). IOP Publishing. https://doi.org/10.1088/1755-1315/679/1/012041

Noersidiq, A., Dilaga, S. H., Suhubdy, S., Amin, M., Fahrullah, F., & Maslami, V. (2023). Urea’s effect on dry matter, organic matter, and crude protein content of ammoniated corn straw. Jurnal Biologi Tropis, 23(4), 76-81. https://doi.org/10.29303/jbt.v23i4.5388

Noersidiq, A., Marlida, Y., Zain, M., Kasim, A., Agustin, F., & Huda, N. (2020). The effect of urea levels on in-vitro digestibility and rumen fermentation characteristic of ammoniated oil palm trunk. International Journal on Advanced Science Engineering Information Technology, 10(3), 1258-1262. https://doi.org/10.18517/ijaseit.10.3.11574

Nurjanah, L. L., Umami, N., Kurniawati, A., Hanim, C., WB, B. P., Paradhipta, D. H. V., & Meidiana, T. (2023, May). The quality of physic and ph of gama umami grass silage supplemented with calliandra leaves and pollard. In IOP Conference Series: Earth and Environmental Science (Vol. 1183, No. 1, p. 012019). IOP Publishing. https://doi.org/10.1088/1755-1315/1183/1/012019

Nuruzzahri, Adam, K. A., Fridayati, D., Koesmara, H. & Ammar, M. (2024). Evaluasi kualitas fisik pakan amoniasi fermentasi (Amofer) limbah jerami padi. Jurnal Peternakan Lokal, 6(2), 53-62. https://doi.org/10.46918/peternakan.v6i2.1967

Ogimoto, K., & Imai., M. (1981). Atlas of Rumen Microbiology. Japan: Japan Scientific Societies Press.

Okoye, C. O., Wang, Y., Gao, L., Wu, Y., Li, X., Sun, J., & Jiang, J. (2023). The performance of lactic acid bacteria in silage production: A review of modern biotechnology for silage improvement. Microbiological Research, 266, 127212. https://doi.org/10.1016/j.micres.2022.127212

Patria, C. A., Riffiandi, N., Pertiwi, V. R., Nurhayati, Usman, N. A., & Priabudiman, Y. (2023). Pengolahan amoniasi jerami padi dengan penambahan urea untuk meningkatkan kecernaan ruminansia. Jurnal Pengabdian Masyarakat, 3(1), 36-40. https://doi.org/10.36728/ganesha.v3i1.2374

Pratiwi, I., Fathul, F., dan Muhtarudin. (2015). Pengaruh penambahan berbagai starter pada pembuatan silase ransum terhadap kadar serat kasar, lemak kasar, kadar air, dan bahan ekstrak tanpa nitrogen silase. Jurnal Ilmiah Peternakan Terpadu, 3(3), 116-120. https://jurnal.fp.unila.ac.id/index.php/JIPT/article/view/835

Qadarullah, M. N., Munir, & Irmayani. (2018). Analisis nilai pH dan tingkat kerusakan silase pakan komplit yang diformulasi dengan daun lamtoro (Leucaena leucocephala) sebagai pakan ternak ruminansia. Jurnal Bionature, 19(2), 119-125. https://doi.org/10.35580/bionature.v19i2.9729

Quirino, D. F., Palma, M. N., Franco, M. O., & Detmann, E. (2023). Variations in methods for quantification of crude ash in animal feeds. Journal of AOAC International, 106(1), 6-13. https://doi.org/10.1093/jaoacint/qsac100

Riswandi, Sandi, S., & Sari, I. P. (2017). Amoniasi fermentasi (Amofer) serat sawit dengan penambahan urea dan effective microorganism-4 (EM-4) terhadap kualitas fisik, derajat keasaman (pH), bahan kering, dan bahan organik. Prosiding Seminar Nasional Lahan Suboptimal. Palembang 19-20 Oktober 2017. http://repository.lppm.unila.ac.id/9356/1/PROSIDING%20SEMINAR%20PALEMBANG.pdf

Sarker, L. R., Khan, M. R. I., & Rahman, M. M. (2018). Ensiling of wet rice straw using biogas slurry and molasses in monsoon of Bangladesh. Journal of Animal Sciences and Livestock Production, 2(1), 1-5. https://doi.org/10.21767/2577-0594.100012

Sharma, R., Garg, P., Kumar, P., Bhatia, S. K., & Kulshrestha, S. (2020). Microbial fermentation and its role in quality improvement of fermented foods. Fermentation, 6(4), 106. https://doi.org/10.3390/fermentation6040106

Sio, S., Bira, G. F., Batu, M. S., Pardosi, L., Mau, R. J., Klau, M. O., & Hoar, J. (2022). Organoleptic quality and nutrition of rice straw silage utilizing local microorganisms (MOL) of cattle rumen fluid at different inoculum levels. Journal of Advanced Veterinary Research, 12(1), 36-41. https://advetresearch.com/index.php/AVR/article/view/832

Suningsih, N., Ibrahim, W., Liandris, O., & Yulianti, R. (2019). Kualitas fisik dan nutrisi jerami padi fermentasi pada berbagai penambahan starter. Jurnal Sain Peternakan Indonesia, 14(2), 191-200. https://doi.org/10.31186/jspi.id.14.2.191-200

Superianto, S., Harahap, A.E., & Ali, A. (2018). Nilai nutrisi silase limbah sayur kol dengan penambahan dedak padi dan lama fermentasi yang berbeda. Jurnal Sain Peternakan Indonesia, 13(2), 172-181. https://ejournal.unib.ac.id/jspi/article/view/3723

Suretno, N. D., Adriyani, F. Y., Tambunan, R. D., Diptaningsari, D., Meidaliyantisyah., Zahara., Maryanto, A. (2023). The potency of rice straw for ruminant feed on several rice varieties. BIO Web of Conferences, 99. https://doi.org/10.1051/bioconf/20249901001

Thiet, N., & Ngu, N. T. (2023). Effect of urea treatment and preservation duration on chemical composition of rice straw offer for growing sind crossbred cattle. Livest. Res. Rural Dev, 35(5), 41. https://www.lrrd.org/lrrd35/5/3541nthi.html

Traughber, Z. T., Detweiler, K.B., Price, A.K., Knap, K.E., Harper, T.A., Swanson, K.S., & de Godoy, M.R. (2021). Effect of crude fiber and total dietary fiber on the calculated nitrogen-free extract and metabolizable energy content of various dog foods fed to client-owned dogs with osteoarthritis. American journal of veterinary research, 82(10), 787-794. https://avmajournals.avma.org/view/journals/ajvr/82/10/ajvr.82.10.787.xml

Widowati, T. W., Hamzah, B., Wijaya, A., & Pambayun, R. (2014). Sifat antagonistik Lactobacillus sp B441 dan II442 asal tempoyak terhadap Staphylococcus aureus. Agritech, 34(4), 430-438. https://doi.org/10.22146/agritech.9438

Yuliana, M. (2021). The effect of local microorganism (MOL) as liquid organic fertilizer to the growth of Ipomea reptans Poir. J. Biota, 7(1), 51-56. https://doi.org/10.19109/Biota.v7i1.7010

Yunilas, Y., Mirwhandhono, E., & Efrata. (2024). Evaluasi kualitas larutan mikroorganisme lokal (MOL) berbasis limbah buah dan potensi sebagai bioaktivator. Jurnal Inovasi Hasil Penelitian dan Pengembangan. 4(1), 1-8. https://doi.org/10.51878/knowledge.v4i1.2786

Yunilas, Y., Siregar, A. Z., Mirwhandhono, E., Purba, A., Fati, N., & Malvin, T. (2022). Potensi dan karakteristik larutan Mikroorganisme Lokal (MOL) berbasis limbah sayur sebagai bioaktivator dalam fermentasi. Journal of Livestock and Animal Health, 5(2), 53-59. http://repository.ppnp.ac.id/858/

Yunilas, Y., Warly, L., Marlida, Y., & Riyanto, I. (2018). Evaluation of fiber content based on palm plantation which has fermentation with probiotic MOIYL. Variabel, 1(1), 18-25. https://dx.doi.org/10.26737/var.v1i1.513

Yusriani, Y., Elviwirda, E., & Sabri, M., 2015. Kajian pemanfaatan limbah jerami sebagai pakan ternak sapi di Provinsi Aceh. Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science), 17(2), 163-169. https://doi.org/10.25077/jpi.17.2.163-169.2015

Zhao, J., Wang, S., Dong, Z., Li, J., Jia, Y., & Shao, T. (2021). Effect of storage time and the level of formic acid on fermentation characteristics, epiphytic microflora, carbohydrate components and in vitro digestibility of rice straw silage. Animal bioscience, 34(6), 1038. https://doi.org/10.5713/ajas.20.0388

Zhou, S., Zhu, Z., Chen, X., Pu, Q., Liu, S., Luo, Y., ... & Wang, X. (2025). Study on the effects of urea addition on the fermentation quality, nitrogen metabolism, microbial community, and metabolic characteristics of cotton strawlage. Frontiers in Microbiology, 16, 1610850. https://doi.org/10.3389/fmicb.2025.1610850

Published
2025-12-08
How to Cite
Ramadhani, R., Apriliana, T. W., Putri, D. A., Amanda, R. A., Shofiah, M., Adeliza, M. F. H., Kurniawan, E., Suharti, S., & Amirroenas, D. E. (2025). KUALITAS FISIK DAN NUTRIEN JERAMI PADI YANG DIFERMENTASI MIKROORGANISME LOKAL ASAL JERAMI PADI. Agrinimal Jurnal Ilmu Ternak Dan Tanaman, 14(1), 13-22. https://doi.org/10.30598/ajitt.2026.14.1.13-22