KEMAMPUAN ANTAGONIS BAKTERI ENDOFIT ASAL TANAMAN SAGU (Metroxylon spp) DALAM MENEKAN PERTUMBUHAN Rhizoctonia solani Kuhn. SECARA IN VITRO

  • Ch. Leiwakabessy Program Studi Agroekoteknologi Fakultas Pertanian Universitas Pattimura
  • Yatni Yatni Alumni Fakultas Pertanian Universitas Pattimura
  • C. Uruilal Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Antakusuma
  • R. E. Ririhena Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Antakusuma
  • F. J. Rumalatu Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Pattimura
Keywords: antagonistic, endophytic bacteria, Rhizoctonia solani, Metroxylon spp

Abstract

Endophytic bacteria is microbe lives in plant tissues without causing symptoms of disease. The benefit of utilizing endophytic bacteria as a technique for controlling diseases such as sprout (R. solani) has been known due to its ability to live and to colonize plant internal tissues. This study aims to determine the ability of endophytic bacteria from sago to suppress the growth of R. solani in vitro. This research was conducted using 19 isolates of endophytic bacteria from sago with 5 replications. The results showed that two endophytic isolates namely STA2 and STA13 were able to suppress the growth of R. solani pathogens compared to controls with inhibition percentage of 41.86% and 16.27%, respectively.

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References

Aravind, R., D. Antony, S. Eapen, K. Aundy, and K. V. Ramana. 2009. Isolation and evaluation of endophytic bacteria against plant parasitic nematodes infesting black pepper (Piper nigrum L.). Indian Journal of Nematology. 39: 211-217.

Balosi, F., I. Lakani, dan J. Panggeso. 2014. Eksplorasi Bakteri Endofit sebagai Agen Pengendalian Hayati terhadap Penyakit Darah pada Tanaman Pisang secara In Vitro. e-J. Arotekbis. 2 (6): 579-586.

Borges, A. A., and L. N. Sandalio. 2015. Induced resistance for plant defense. Frontiers in plant science. 6: 109.

Chebotar, V.K., N.V Malfanova, A.V. Shcherbakov, et al. 2015. Appl Biochem Microbiol. 51: 271. https://doi.org/10.1134/S0003683815030059.

Cipollini, D., C. M. Rigsby, and E. K. Barto. 2012. Microbes as Targets and Mediators of Allelopathy in Plants. Journal of chemical ecology. 38: 714-27.

Compant, S., C. Clément, and A. Sessitscha. 2010. Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology & Biochemistry. 42: 669-678.

Fravel, D.R. 2003. Role of Antibiosis In the Biocontrol of Plant Diseases. Annual Review of Phytopathology. 26(1): 75-91.

Hariprasad, P., S. Divakara, and S. Niranjana. 2011. Isolation and characterization of chitinolytic bacteria for the management of Fusarium wilt in tomato. Crop Protect. 30(12):1606-1612.

Harni, R., dan M.S.D. Ibrahim. 2011.Potensi Bakteri Endofit Menginduksi Ketahanan Tanaman Lada Terhadap Infeksi Meloidogyne incognita. Balai Penelitian Tanaman Rempah dan Aneka Tanaman Industri. Jurnal Littri. 17: 118–123.

Herdyastuti, N., T.J. Raharjo, Mudasir and S. Matsjeh. 2009. Chitinase and Chitinolityc Microorganism : Isolation, Characterization and Potential. Indo.J.Chem. 9(1): 37-47.

Leiwakabessy, Ch., M.S. Sinaga, K.H. Mutaqien, Trikoesoemaningtyas, and Giyanto. 2018. The endophytic bacteria, salicylic acid, and their combination as inducers of rice resistance against Xanthomonas oryzae pv. oryzae. AGRIVITA Journal of Agricultural Science. 40(1): 25–35.

Mei, C., and B. Flinn. 2009. The Use of Beneficial Microbial Endophytes for Plant Biomass and Stress Tolerance Improvement. Recent patents on biotechnology.

Melliawati, R., H.I. Sukiman, D.N. Widyaningrum, F. Octavina, E. Sukmawati, and P. Simanjuntak. 2004. Studies on Indonesian endophytic microorganism and their potentials for plant protection of pathogen bacteria. Indonesian Biotechnology Conference. Konsorsium Bioteknologi Indonesia, Sanur, Bali, December 1-3rd, 2004.

Melliawati .R., D.N. Widyaningrum, A.C. Djohan, dan H. Sukiman. 2006. Pengkajian Bakteri Endofit Penghasil Senyawa Bioaktif Untuk Proteksi Tanaman. Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia(LIPI). Biodeversitas. 7: 221-224.

Miliute, I., O. Buzaite, D. Baniulis, and V. Stanys. 2015. Bacterial endophytes in agricultural crops and their role in stress tolerance : a review. Zemdirbyste-Agriculture. 102(4): 465–478.

Mohamad, O., L. Li, J.B. Ma, S. Hatab, L. Xu, J.W. Guo, and W.J. Li. 2018. Evaluation of the Antimicrobial Activity of Endophytic Bacterial Populations From Chinese Traditional Medicinal Plant Licorice and Characterization of the Bioactive Secondary Metabolites Produced by Bacillus atrophaeus Against Verticillium dahliae. Frontiers in microbiology. 9: 924.

Pusey, P. & Stockwell, Virginia & Reardon, Catherine & Smits, Theo & Duffy, Brion. 2011. Antibiosis Activity of Pantoea agglomerans Biocontrol Strain E325 Against Erwinia amylovora on Apple Flower Stigmas. Phytopathology. 101: 1234-1241.

Rosenblueth, M., and E. Martinez-Romero. 2006. Bacterial Endophytes and Their Interactions with Hosts. Molecular Plant-Microbe Interactions. 19: 827-837.

Santoyo, G., G. Moreno-Hagelsieb, M. C. Orozco-Mosqueda, and BR. Glick. Plant growth-promoting bacterial endophytes. 2016. Microbiol Res. 183: 92-99.

Sessitsch, A., T. Coenye, A.V. Sturz, P. Van damme, E.A. Barka, J.F. Salles, and J. Nowak. 2005. Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties. Int J Syst Evol Microbiol. 55(3): 1187-1192.

Skidmore, A.M., and C.H. Dickinson. 1976. Colony interactions and hyphal interference between Septoria nodorum and phylloplane fungi. Trans Brit Mycol Soc. 66: 57-64.

Tezerji S, Raheleh & Rattei, Thoms & Sessitsch, Angela & Trognitz, Friederike & Mitter, Birgit. 2015. Transcriptome Profiling of the Endophyte Burkholderia phytofirmans PsJN Indicates Sensing of the Plant Environment and Drought Stress. mBio. 6.e00621-15.

Wulandari, H., Zakiyatulyaqin dan Supriyanto. 2012. Isolasi dan pengujian bakteri endofit dari tanaman lada (Piper ningrum L.) sebagai antagonis terhadap patogen hawar beludru (Septobasidium sp.). Jurnal Perkebunan dan Lahan Tropika. 2(2): 23-31.

Zou, Y., Q. Yu, Y.H. Chiu, and X. Bi. 2006. Position effect on the directionality of silencer function in Saccharomyces cerevisiae. Genetics. 174(1): 203–213.

Published
2019-10-31
How to Cite
Leiwakabessy, C., Yatni, Y., Uruilal, C., Ririhena, R. E., & Rumalatu, F. J. (2019). KEMAMPUAN ANTAGONIS BAKTERI ENDOFIT ASAL TANAMAN SAGU (Metroxylon spp) DALAM MENEKAN PERTUMBUHAN Rhizoctonia solani Kuhn. SECARA IN VITRO. Agrinimal Jurnal Ilmu Ternak Dan Tanaman, 7(2), 48-52. https://doi.org/10.30598/ajitt.2019.7.2.48-52