POTENSI PENINGKATAN AKTIVITAS ANTIOKSIDAN YOGURT MELALUI FORTIFIKASI EKSTRAK BUAH, REMPAH, DAN BERBAGAI JENIS TEH: REVIEW

POTENTIAL ENHANCEMENT OF ANTIOXIDANT ACTIVITY IN YOGURT THROUGH FORTIFICATION OF FRUIT EXTRACTS, SPICES, AND VARIOUS TYPES OF TEA: A REVIEW

  • Irfan Fadhlurrohman Program Studi Teknologi Hasil Ternak, Fakultas Peternakan, Universitas Jenderal Soedirman, Indonesia
  • Muhammad Iqbal Maulana Program Studi Teknologi Hasil Ternak, Fakultas Peternakan, Universitas Jenderal Soedirman, Indonesia
  • Regina Ananda Pratiwi Program Studi Teknologi Hasil Ternak, Fakultas Peternakan, Universitas Jenderal Soedirman, Indonesia
  • Robiul Akhwal Program Studi Teknologi Hasil Ternak, Fakultas Peternakan, Universitas Jenderal Soedirman, Indonesia
  • Triana Setyawardani Program Studi Teknologi Hasil Ternak, Fakultas Peternakan, Universitas Jenderal Soedirman, Indonesia
  • Juni Sumarmono Program Studi Teknologi Hasil Ternak, Fakultas Peternakan, Universitas Jenderal Soedirman, Indonesia
Keywords: antioxidant activity, lactic acid bacteria, fortification, natural ingredients, yogurt

Abstract

Yogurt merupakan produk pangan fungsional yang mengandung bakteri asam laktat (BAL) dan berbagai senyawa bioaktif hasil fermentasi, namun aktivitas antioksidannya masih dapat ditingkatkan melalui fortifikasi bahan alami. Artikel ini bertujuan untuk mengkaji pengaruh penambahan berbagai bahan fortifikasi, meliputi buah, rempah, dan teh terhadap aktivitas antioksidan yogurt dan mengevaluasi pengaruhnya terhadap viabilitas BAL. Metode yang digunakan adalah studi literatur dengan menelusuri artikel ilmiah yang diterbitkan pada tahun 2016–2026 melalui Google Scholar, ScienceDirect, dan ResearchGate. Artikel yang relevan kemudian dianalisis secara deskriptif dengan membandingkan jenis bahan, konsentrasi fortifikasi, peningkatan aktivitas antioksidan, serta pengaruhnya terhadap BAL. Hasil kajian menunjukkan bahwa fortifikasi buah, teh, dan rempah secara umum mampu meningkatkan aktivitas antioksidan yogurt, tetapi besarnya peningkatan berbeda bergantung pada jenis dan konsentrasi bahan. Fortifikasi buah seperti bit, buah naga, dan alpukat dilaporkan menghasilkan peningkatan aktivitas antioksidan hingga 97,94%, sedangkan teh, terutama teh hijau dan teh hitam, menunjukkan peningkatan hingga 96,87% yang berkaitan dengan kandungan polifenol. Rempah seperti jahe, kayu manis, dan lada hitam juga berkontribusi terhadap peningkatan aktivitas antioksidan melalui kandungan senyawa fenolik dan flavonoid. Peningkatan aktivitas antioksidan tersebut umumnya terjadi pada konsentrasi fortifikan tertentu, sedangkan penggunaan konsentrasi yang terlalu tinggi berpotensi menurunkan penerimaan sensoris dan menghambat viabilitas BAL. Secara keseluruhan, fortifikasi bahan alami berpotensi meningkatkan aktivitas antioksidan yogurt tanpa mengganggu viabilitas BAL apabila digunakan pada konsentrasi yang sesuai. Pemilihan jenis dan konsentrasi fortifikan menjadi faktor penting dalam menghasilkan yogurt dengan aktivitas antioksidan tinggi, viabilitas BAL yang tetap baik, dan kualitas produk yang dapat diterima.

ABSTRACT 

Yogurt is a functional food product containing lactic acid bacteria (LAB) and various bioactive compounds derived from fermentation; however, its antioxidant activity can be further enhanced through fortification with natural ingredients. This article aims to review the impact of adding various fortifying agents—including fruits, tea, and spices—on the antioxidant activity of yogurt and to evaluate their effects on LAB viability. A literature review method was employed, involving a search for scientific articles published between 2016 and 2026 via Google Scholar, ScienceDirect, and ResearchGate. Relevant articles were analyzed descriptively by comparing ingredient types, fortification concentrations, increases in antioxidant activity, and effects on LAB. The review results indicate that fortification with fruits, tea, and spices generally enhances yogurt's antioxidant activity, although the magnitude of this increase varies depending on the type and concentration of the ingredient. Fortification with fruits such as beetroot, dragon fruit, and avocado has been reported to increase antioxidant activity by up to 97.94%, while tea—particularly green and black tea—showed increases of up to 96.87%, attributed to polyphenol content. Spices such as ginger, cinnamon, and black pepper also contribute to increased antioxidant activity through their phenolic and flavonoid content. These increases in antioxidant activity generally occur at specific fortificant concentrations; conversely, excessively high concentrations may reduce sensory acceptability and inhibit LAB viability. Overall, fortification with natural ingredients has the potential to enhance yogurt's antioxidant activity without compromising LAB viability, provided appropriate concentrations are used. The selection of the type and concentration of the fortifying agent is a crucial factor in producing yogurt with high antioxidant activity, maintained LAB viability, and acceptable product quality.

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References

Ahmad, I., Hao, M., Li, Y., Zhang, J., Ding, Y., & Lyu, F. (2022). Fortification of yogurt with bioactive functional foods and ingredients and associated challenges-A review. Trends in food science & technology, 129, 558-580. https://doi.org/10.1016/j.tifs.2022.11.003

Aisy, N. R., Suwita, I. K., & Razak, M. (2025). Pengaruh penambahan yogurt dan jeruk nipis terhadap aktivitas antioksidan dan mutu organoleptik jus bit (Beta vulgaris L.) untuk penyakit tidak menular. Nutriture Journal, 4 (1), 10–19. https://ojs.poltekkes-malang.ac.id/Nutriture/article/view/4744

Amalia, R. N., Rahmiwati, A., Fajar, N. A., & Sari, N. (2024). Preferensi pangan fungsional dan implikasinya terhadap kesehatan masyarakat: Tinjauan sistematis. Jurnal SAGO Gizi dan Kesehatan, 5(2), 456-465. http://dx.doi.org/10.30867/gikes.v5i2.1654

Amaliah, P., Yuningtyas, S., & Noviardi, H. (2025). Aktivitas antioksidan dan karakteristik dari yoghurt sari kedelai dengan penambahan sari jahe merah (Zingiber officinale var. rubrum). Jurnal Farmamedika (Pharmamedica Journal), 10 (1)(1), 107–113. https://doi.org/10.47219/ath.v10i1.446

Ameilia, Somantara, I. G. B., & Hasanah, A. N. (2022). Review: potential of synbiotic yoghurt with spice combination as probiotic and antioxidant supplementation based on nutraceutical natural ingredients from lontar usadha taru pramana as local medical wisdom of Bali. Journal of Pharmaceutical and Sciences, 6 (1), 152–159. https://doi.org/10.36490/journal-jps.com.v6i1.44

Ardani, N. F., Pramudita, F. G., Fitriyani, & Fadhlurrohman, I. (2025). Fortifikasi ekstrak bunga rosella (Hibiscus sabdariffa L.) pada produk olahan susu sebagai pangan fungsional. Prosiding Seminar Nasional Pembangunan Dan Pendidikan Vokasi Pertanian, 6(1), 699–710. https://doi.org/10.47687/snppvp.v6i1.1818

Arkan, N. D. A., & Setyawardani, T. (2025). Functional Potential of Greek Yoghurt as a Fermented Dairy Product: A literature review. Review. Jurnal Ilmiah AgriSains, 26(3), 153-162. https://doi.org/10.22487/jiagrisains.v26i3.2025.153-162

Benozzi, E., Romano, A., Capozzi, V., Makhoul, S., Cappellin, L., Khomenko, I., Aprea, E., Scampicchio, M., Spano, G., Märk, T. D., Gasperi, F., & Biasioli, F. (2015). Monitoring of lactic fermentation driven by different starter cultures via direct injection mass spectrometric analysis of flavour-related volatile compounds. Food Research International, 76, 682–688. https://doi.org/10.1016/j.foodres.2015.07.043

Buniowska-Olejnik, M., Urbański, J., Mykhalevych, A., Bieganowski, P., Znamirowska-Piotrowska, A., Kačániová, M., & Banach, M. (2023). The influence of curcumin additives on the viability of probiotic bacteria, antibacterial activity against pathogenic microorganisms, and quality indicators of low-fat yogurt. Frontiers in Nutrition, 10. https://doi.org/10.3389/fnut.2023.1118752

Celik, O. F., Kilicaslan, M., Akcaoglu, S., & Ozturk, Y. (2023). Improving the antioxidant activity of yogurt through black and green tea supplementation. International Journal of Food Science and Technology, 58(11), 6121-6130. https://doi.org/10.1111/ijfs.16722

Christin, M., Setyawardani, T., & Ifani, M. (2025). Nilai pH dan keasaman es krim yoghurt dengan penambahan persentase bubuk kayu secang (Caesalpinia sappan L.) yang berbeda. ANGON: Journal of Animal Science and Technology, 7(1), 1-9. http://jnp.fapet.unsoed.ac.id/index.php/angon/id/article/view/1345

Edkaidek, H., Dahiya, D., & Nigam, P. S. (2026). Prospects of bioactive compounds in designing functional foods: Challenges and solutions. Foods, 15(8), 1291. https://doi.org/10.3390/foods15081291

Fadhlillah, M., Fahruddin, M. F., Anggraeni, N. I., Ginting, S. D. B., Soedjanaatmadja, U. M., & Ishmayana, S. (2024). Evaluasi kandungan polifenol dan profil nutrien yogurt dengan penambahan kulit buah naga merah (Hylocereus polyrhizus). Kimia Padjadjaran, 3(1), 30-39. https://jurnal.unpad.ac.id/jukimpad/article/view/66011

Fadhlurrohman, I., & Susanto, J. (2024). Functional food innovation based on fermented milk products with fortification of various types of tea: A review. JITIPARI (Jurnal Ilmiah Teknologi dan Industri Pangan UNISRI), 9(1), 101-114. https://doi.org/10.33061/jitipari.v9i1.10221

Frilanda, A., Putranto, W. S., & Gumilar, J. (2022). Pengaruh berbagai konsentrasi pulp buah naga merah pada pembuatan set yoghurt terhadap total bakteri asam laktat, nilai pH, dan total asam. Jurnal Teknologi Hasil Peternakan, 3(1)(1), 32–41. https://doi.org/10.24198/jthp.v3i1.39239

Hassanpour, S. H., & Doroudi, A. (2023). Review of the antioxidant potential of flavonoids as a subgroup of polyphenols and partial substitute for synthetic antioxidants. Avicenna journal of phytomedicine, 13(4), 354. https://doi.org/10.22038/AJP.2023.21774

Hartati, Hilmi, I., Nur, D., Awaludin, K., Fadhlurrohman, I., Arkan, N. D., Sumarmono, J., & Setyawardani, T. (2025). Fortifikasi teh rosella ungu (Hibiscus sabdariffa L.) sebagai pewarna alami terhadap sifat fisikokimia dan organoleptik yogurt. Jurnal Triton, 16(1), 188–198. https://doi.org/https://doi.org/10.47687/jt.v16i1.936

Helkar, B. P., & Sahoo, A. (2016). Review: food industry by-products used as functional food ingredients. International Journal of Waste Resources, 6(3). https://doi.org/10.4172/2252-5211.1000248

Horiuchi, H., Inoue, N., Liu, E., Fukui, M., Sasaki, Y., & Sasaki, T. (2009). A method for manufacturing superior set yogurt under reduced oxygen conditions. Journal of Dairy Science, 92(9), 4112–4121. https://doi.org/10.3168/jds.2008-1747

Hussain, Y., Luqman, S., & Meena, A. (2020). Research progress in flavonoids as potential anticancer drug including synergy with other approaches. Current Topics in Medicinal Chemistry, 20(20), 1791–1809. https://doi.org/10.2174/1568026620666200502005411

Huwae, L. M. C., Alfons, M. A., Tanikwele, V. C., Linansera, K. J., & Linansera, P. E. (2022). Pengaruh penambahan wine pala terhadap aktivitas antioksidan dan antibakteri yoghurt sari buah sirsak (Annona muricata L.). Molucca Medica, 15(2), 110-117. https://doi.org/10.30598/molmed.2022.v15.i2.110

Jaffar, H. M., Ambreen, S., Al-Asmari, F., Hussain, I., Rahim, M. A., Ramadan, M. F., ... & Zongo, E. (2024). Antioxidant activity, microbial viability, and sensory attributes of traditional-yogurt enriched with silymarin. Cogent Food Agric, 10(1), 1–17. https://doi.org/10.1080/23311932.2024.2417838

Jeong, C. H., Ryu, H., Zhang, T., Lee, C. H., Seo, H. G., & Han, S. G. (2018). Green tea powder supplementation enhances fermentation and antioxidant activity of set-type yogurt. Food Science and Biotechnology, 27(5), 1419–1427. https://doi.org/10.1007/s10068-018-0370-9

Jiménez-Redondo, N., Vargas, A. E., Teruel-Andreu, C., Lipan, L., Muelas, R., Hernández-García, F., ... & Cano-Lamadrid, M. (2022). Evaluation of cinnamon (Cinnamomum cassia and Cinnamomum verum) enriched yoghurt during refrigerated storage. Lwt-Food Science and Technology, 159, 113240. https://doi.org/10.1016/j.lwt.2022.113240

Kalogerakou, T., & Antoniadou, M. (2024). The role of dietary antioxidants, food supplements and functional foods for energy enhancement in healthcare professionals. Antioxidants, 13(12), 1508. https://doi.org/10.3390/antiox13121508

Kusuma, B. A. D., Aminah, S., & Harsoelistyorini, W. (2022). Aktivitas antioksidan, karakteristik fisik, dan sensoris yogurt beku kecambah kacang merah dengan variasi penambahan ekstrak kulit buah naga merah. Jurnal Pangan Dan Gizi, 12(1), 32. https://doi.org/10.26714/jpg.12.1.2022.32-40

Kusumayanti, H., Mahendrajaya, R. T., & Hanindito, S. B. (2018). Pangan fungsional dari tanaman lokal Indonesia. Jurnal Metana, 12(01), 26–30. https://ejournal.undip.ac.id/index.php/metana/article/view/17512

Lazaridis, D. G., Kitsios, A. P., Koutoulis, A. S., Malisova, O., & Karabagias, I. K. (2024). Fruits, spices and honey phenolic compounds: a comprehensive review on their origin, methods of extraction and beneficial health properties. Antioxidants, 13(11), 1335. https://doi.org/10.3390/antiox13111335

Liu, D., Yang, M., Sun, Y., & Zeng, X. (2026). Effect of heat pre-treatment on interaction between micellar casein and betanin, and characteristics of their complexes. Food Chemistry: X, 37(March), 104135. https://doi.org/10.1016/j.fochx.2026.104135

Maharani, A. I., Riskierdi, F., Febriani, I., Kurnia, K. A., Rahman, N. A., Ilahi, N. F., & Farma, S. A. (2021). Peran antioksidan alami berbahan dasar pangan lokal dalam mencegah efek radikal bebas. In Prosiding Seminar Nasional Biologi, 1(2), 390-399. https://semnas.biologi.fmipa.unp.ac.id/index.php/prosiding/article/view/355

Maharani, S., Sugiarti, Y., Al Falah, S. A. N. W., & Prawira-Atmaja, M. I. (2021). Kadar polifenol dan aktivitas antioksidan yoghurt teh dengan inokulum Caspian Sea selama fermentasi. Jurnal Ilmu Pangan dan Hasil Pertanian, 5(2), 181-190. https://doi.org/10.26877/jiphp.v5i2.10221

Maleta, H. S., & Kusnadi, J. (2018). Pengaruh penambahan sari buah naga merah (Hylocereus polyrhizus) terhadap aktivitas antioksidan dan karakteristik fisikokimia Caspian Sea yoghurt. Jurnal Pangan dan Agroindustri, 6(2), 13–22. https://doi.org/10.21776/ub.jpa.2018.006.02.2

Mbaeyi-Nwaoha, I. E., Uzoigwe, A. C., & Onyeaka, H. (2024). Quality evaluation of herbal yoghurt produced using cinnamon (Cinnamon cassia), garlic (Allium sativum), and ginger (Zingiber officinale). Journal of Food Quality, 2024(1). https://doi.org/10.1155/2024/8262211

Midah, Z., Fajriansyah, F., Makmun, A., & Rasfahyana, R. (2021). Hubungan obesitas dan stress oksidatif. UMI Medical Journal, 6(1), 62-69. https://doi.org/10.33096/umj.v6i1.140

Montemurro, M., Pontonio, E., Coda, R., & Rizzello, C. G. (2021). Plant-based alternatives to yogurt: State-of-the-art and perspectives of new biotechnological challenges. Foods, 10(2), 316. https://doi.org/10.3390/foods10020316

Muniandy, P., Shori, A. B., & Baba, A. S. (2016). Influence of green, white and black tea addition on the antioxidant activity of probiotic yogurt during refrigerated storage. Food Packaging and Shelf Life, 8, 1–8. https://doi.org/10.1016/j.fpsl.2016.02.002

Munteanu-Ichim, R. A., Canja, C. M., Lupu, M., Bădărău, C. L., & Matei, F. (2024). Tradition and Innovation in Yoghurt from a Functional Perspective—A Review. Fermentation, 10 (7), 357. https://doi.org/10.3390/fermentation10070357

Ningtiyas, A. R., Dewi, G. P., Ridwan, M., Ramadhani, N. S., & others. (2025). Analisis pengendalian kualitas produksi yogurt di UMKM Susu Segar Sukalarang. Jurnal Teknologi Dan Manajemen Industri Terapan, 4(2), 378–386. https://doi.org/10.55826/jtmit.v4i2.639

Putra, L. A. G., Krisbianto, O., & Brotosudarmo, T. H. P. (2024). A review of the effects of fruit extracts fortification on the quality characteristics and antioxidant activities of yogurt. Jurnal Teknologi Hasil Pertanian, 17(2), 147–159. https://jurnal.uns.ac.id/ilmupangan/article/view/61941

Rahmi, H. (2017). Aktivitas antioksidan dari berbagai sumber buah-buahan di Indonesia. Jurnal Agrotek Indonesia, 2(1), 34-38. https://doi.org/10.33661/jai.v2i1.721

Ramadhan, A. D., Maslachah, L. U. W., Nurjannah, D. A. K., Setyawardani, T., Sumarmono, J., Fadhlurrohman, I., & Arkan, N. D. (2024). Sifat Fisikokimia dan Organoleptik Yoghurt dengan Fortifikasi Teh Pandan (Pandanus amaryllifolius Roxb.). Jurnal Ilmiah Peternakan Halu Oleo, 6(4), 336-344. https://doi.org/10.56625/jipho.v6i4.67

Renzetti, A., Betts, J. W., Fukumoto, K., & Rutherford, R. N. (2020). Antibacterial green tea catechins from a molecular perspective: mechanisms of action and structure–activity relationships. Food & Function, 11(11), 9370–9396. https://doi.org/10.1039/d0fo02054k

Rosiana, N. M., & Khoiriyah, T. (2018). Yogurt tinggi antioksidan dan rendah gula dari sari buah apel Rome Beauty dan madu. Jurnal Ilmu Dan Teknologi Hasil Ternak, 13(2), 81–90. https://doi.org/10.21776/ub.jitek.2018.013.02.2

Sari, F. Y. K., Septiani, S., & Melati, A. (2024). Karakteristik organoleptik dan kimia produk yogurt sinbiotik dari umbi lokal. Jurnal Medika Indonesia, 5(1), 9–18. https://ejr.umku.ac.id/index.php/JMI/article/view/2253

Shori, A. B. (2020). Inclusion of phenolic compounds from different medicinal plants to increase α-amylase inhibition activity and antioxidants in yogurt. Journal of Taibah University for Science, 14(1), 1000–1008. https://doi.org/10.1080/16583655.2020.1798072

Shori, A. B. (2022). Storage quality and antioxidant properties of yogurt fortified with polyphenol extract from nutmeg, black pepper, and white pepper. Electronic Journal of Biotechnology, 57, 24-30. https://doi.org/10.1016/j.ejbt.2022.03.003

Sibuea, P., & Siantar, V. N. L. (2022). Aktivitas antioksidan yoghurt dengan penambahan ekstrak buah bit (Beta vulgaris L.) dan mutu probiotik yang dihasilkan. Jurnal Riset Teknologi Pangan Dan Hasil Pertanian (RETIPA), 3(1), 22-29.. https://doi.org/10.54367/retipa.v3i1.2243

Suciati, F., & Safitri, L. S. (2021). Pangan fungsional berbasis susu dan produk turunannya. Journal of Surimi (Sustainable Research In Management of Agroindustry), 1(1), 13-19. https://doi.org/10.35970/surimi.v1i1.535

Supriyanti, F. M. T., Rahayu, S., & Sholihin, H. (2021). Karakteristik fisikokimia dan aktivitas antioksidan yoghurt terfortifikasi tanaman obat dengan kandungan antioksidan tinggi. Chemica Isola, 2(2), 108-116. https://ejournal.upi.edu/index.php/CI/article/view/FMTS

Tena, N., Martín, J., & Asuero, A. G. (2025). State of the art of anthocyanins: Antioxidant activity, sources, bioavailability, and therapeutic effect in human health. Handbook of Analysis and Extraction Methods of Anthocyanins, 3–33. https://doi.org/10.1201/9781003453260-2

Wulan, D. F. P. (2024). Yogurt susu kambing Etawa (Capra aegagrus hircus) dengan penambahan daging buah alpukat (Persea americana Mill.) sebagai minuman fungsional. Jurnal Natural Scientiae, 4(2), 47–64. https://doi.org/10.20527/jns.v4i2.13316

Published
2026-09-01
How to Cite
Fadhlurrohman, I., Maulana, M. I., Pratiwi, R. A., Akhwal, R., Setyawardani, T., & Sumarmono, J. (2026). POTENSI PENINGKATAN AKTIVITAS ANTIOKSIDAN YOGURT MELALUI FORTIFIKASI EKSTRAK BUAH, REMPAH, DAN BERBAGAI JENIS TEH: REVIEW. Agrinimal Jurnal Ilmu Ternak Dan Tanaman, 14(2), 201-212. https://doi.org/10.30598/ajitt.2026.14.2.201-212