Uji Aktivitas Antibakteri Vibrio sp. dengan Ekstrak Etanol Daun Sirsak (Annona muricata L.)

  • Anggreni Sianturi Program Studi Bioteknologi Perikanan, Jurusan Teknologi Hasil Perikanan, Politeknik Perikanan Negeri Tual
  • Hendro Hitijahubessy Program Studi Bioteknologi Perikanan, Jurusan Teknologi Hasil Perikanan, Politeknik Perikanan Negeri Tual
  • Anastasia Putri Marshanda Rumangun Program Studi Bioteknologi Perikanan, Jurusan Teknologi Hasil Perikanan, Politeknik Perikanan Negeri Tual
  • Aprianti Samid Program Studi Bioteknologi Perikanan, Jurusan Teknologi Hasil Perikanan, Politeknik Perikanan Negeri Tual
DOI: https://doi.org/10.30598/biofaal.v4i2pp072-080
Keywords: Soursop leaves, Vibrio sp., Phytochemical

Abstract

Study of the antibacterial ability of soursop leaf extract (Annona muricata L.) in inhibiting the growth of Vibrio sp. The maceration method with 70% ethanol solvent was used in this research to determine the yield, then the extract was diluted in stages from a concentration of 100% to 3.125%. The antibacterial test was done using the disc diffusion method in TCBS media and a phytochemical test was carried out on soursop leaves to analyze the secondary metabolite content. The extract yield obtained in this study was 3.77%. The test results showed that the largest zone of inhibition was found at 100% extract concentration of 11.45mm. The smallest inhibition zone was found in the 6.25% extract with an inhibition zone of 1.44mm, the diameter of the positive control (tetracycline) inhibition zone was 22.75mm, the diameter of 10% DMSO as a negative control was 0 mm. Phytochemical tests of soursop leaves show that they contain alkaloids, flavonoids, steroids, saponins and tannins.

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Published
2023-12-01
How to Cite
Sianturi, A., Hitijahubessy, H., Rumangun, A., & Samid, A. (2023). Uji Aktivitas Antibakteri Vibrio sp. dengan Ekstrak Etanol Daun Sirsak (Annona muricata L.). Biofaal Journal, 4(2), 072-080. https://doi.org/10.30598/biofaal.v4i2pp072-080
Issue
Vol 4 No 2 (2023): Biofaal Journal
Section
Articles

Copyright (c) 2023 Anggreni Sianturi, Hendro Hitijahubessy, Anastasia Putri Marshanda Rumangun, Aprianti Samid

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