UJI AKTIVITAS ANTIDIABETES EKSTRAK DAUN PACAR AIR (Impatiens balsamina L.) TERHADAP GINJAL TIKUS WISTAR (Rattus novergicus L.) YANG DIINDUKSI ALOKSAN
Abstract
Background: Diabetes mellitus is a serious, "chronic" condition that occurs when there is an increase in a person's blood sugar levels because the body is unable to produce enough insulin hormones/cannot effectively use the insulin produced. A lack of insulin, or the inability of cells to respond to it, causes high blood glucose levels (hyperglycemia), which causes clinical indicators of diabetes.
Methods: This research is an experimental study using a completely randomized design. Twenty-four male white rats were divided into 6 groups consisting of normal controls (KN) who received no treatment, negative controls (K-) who were injected with alloxan at a dose of 150 mg/kg BW, positive controls (K+) who were injected with alloxan at a dose of 150 mg/kg BW. kg BW and glibenclamide at a dose of 0.09 mg/200 g BW, Treatment 1 (P1) was injected with alloxan at a dose of 150 mg/kg BW and ethanol extract of water henna leaves at a dose of 450 mg/KG BW, Treatment 2 (P2) was injected with alloxan with a dose of 150 mg/kg BW and ethanol extract of water henna leaves with a dose of 500 mg/KG BW, Treatment 3 (P3) was injected with alloxan at a dose of 150 mg/kg BW and ethanol extract of water henna leaves with a dose of 550 mg/KG BW. Mice blood glucose levels were measured on days 0 (after alloxan induction), 3, 7, and 14. Before the measurements, the mice were fasted for 8 hours.
Results: Research shows that administration of water henna leaf extract has a significant effect in reducing urea and creatinine levels, as well as improving kidney morphology. However, it could not prevent the alloxan-induced increase in kidney weight in white mice.
Conclusion: Administration of henna leaf extract (Impatiens balsamina L.) at a dose of 500 mg/kg BW had a significant effect on reducing urea and creatinine levels in white rats induced by alloxan. Administration of water henna leaf extract (Impatiens balsamina L.) at a dose of 450 mg/kg BW had a significant effect in improving kidney morphology in alloxan-induced white rats, whereas the organ index of water henna leaf extract could not prevent the increase in kidney weight in white mice induced alloxan
Downloads
References
Dewi, P. R. P., Hairrudin, H., & Normasari, R. (2016). Pengaruh Stres Fisik terhadap Kadar Kreatinin Serum Tikus Wistar Jantan (Rattus norvegicus) (The Effect of Physical Stress on Serum Creatinine of Male Rattus norvegicus). Jurnal Pustaka Kesehatan, 4(2), 219.
Fahrimal, Y., Rahmiwati, R., & Aliza, D. (2016). 26. Gambaran Histopatologis Ginjal Tikus Putih (Rattus novergicus) Jantan Yang Diinfeksikan Trypanosoma Evansi Dan Diberi Ekstrak Daun Sernai (Wedelia biflora) (Histopathology of Male Rat (Rattus novergicus) Kidney Infected with Trypanosoma evansi and Treated with Sernai Leaves Extract (Wedelia biflora)). Jurnal Medika Veterinaria, 10(2), 169.
Farizal, J. (2019). Gambaran Kadar Ureum Pada Pasien penyakit Jantung Koroner Di Ruang Rawat Inap Iccu Rsud Dr. M. Yunus Provinsi Bengkulu. Journal of Nursing and Public Health, 7(1), 73.
Hardiningtyas, S. D., Purwaningsih, S., & Handharyani, E. (2014). Aktivitas antioksidan dan efek hepatoprotektif daun bakau api-api putih. Jurnal Pengolahan Hasil Perikanan Indonesia, 17(1), 84.
Haryoto & Devi, E. S. (2018). Efek Pemberian Ekstrak Etanol Daun Dan Batang Ubi Jalar Ungu (Ipomoea batatas L.) Terhadap Penurunan Kadar Glukosa Darah Pada Tikus Jantan Galur Wistar Yang Diinduksi Aloksan. Journal OF TM Conference Series, (1), 142.
Hasim, H., Faridah, D. N., Safithri, M., Husnawati, H., Setiyono, A., & Manshur, H. A. (2020). Aktivitas Penurunan Kadar Glukosa pada Tikus yang Diinduksi Aloksan dari Ekstrak Air Angkak, Bekatul, dan Kombinasinya. Indonesian Journal of Industrial Research, 37(2), 174.
Hendromartono, 2007. Nefropati Diabetik. Jakarta: Balai Penerbit FK UI.
Ichsan, M. Z., Febriani, H., & Syukriah, S. (2022). Efek Pemberian Ekstrak Etanol Rimpang Jeringau Terhadap Gambaran Morfohistologi Ginjal Tikus Pasca Induksi Natrium Nitrit. Klorofil: Jurnal Ilmu Biologi Dan Terapan , 6 (2), 4-5.
IDF, L D. F. (2021). IDF Diabetes Atlas, 10th Edition. Iternational Diabetes Federation.
Ih, Hariyanto, Fajriaty, I., Wijaya, T., & Hafizh, Muhammad. (2018). The potential ethnomedicine plant of Impatiens balsamina leaves from Pontianak, West Kalimantan, Indonesia for wound healing. Journal of Nusantara Bioscience, 10(1), 58.
Mauruh, D. N., Tandi, J., Dewi, N. P., & Yusriadi, Y. (2019). Pengaruh Ekstrak Etanol Daun Kacang Terhadap Kadar Ureum Kreatinin Tikus Putih Yang Diinduksi Streptozotocin. Farmakologika: Jurnal Farmasi, 16(02), 146-150.
Melisa, E., & Yuliawati, Y. (2022). Uji toksisitas akut ekstrak etanol daun sungkai (Peronema cenescens Jack) terhadap fungsi ginjal mencit putih betina (Mus musculus Linn.). Majalah Farmasi dan Farmakologi, 26(1), 35.
Muqsita, V., Sakinah, E. N., & Santosa, A. (2015). Efek Ekstrak Etanol Kayu Manis (Cinnamomum burmannii) terhadap Kadar MDA Ginjal pada Tikus Wistar Hiperglikemi. E-Jurnal Pustaka Kesehatan, 3(2), 235-238.
Oktaviani, D., Yuniastuti, A., & Christijanti, W. (2021). Aktivitas Antioksidan Dari Pati Umbi Gembili (Dioscorea esculenta L.) Pada Tikus Hiperkolestrolemia. In Prosiding Seminar Nasional Biologi (9), 175.
Pasaribu, W., & Longdong, S. N. (2015). Efektivitas Ekstrak Daun Pacar Air (Impatiens balsamina L.) untuk Meningkatkan Respon Imun Non Spesifik Ikan Nila (Oreochromis niloticus). E-Journal Budidaya Perairan, 3(1), 84.
Putri, N. F., Lyrawati, D., & Sarwono, I. (2015). Efek Asam Alfa Lipoat Pada Kadar MDA Dan Histologi Ginjal Tikus Wistar Diabetes Melitus Tipe1. Jurnal Kedokteran Brawijaya, 28(3), 180.
Sayekti, N. C. N., & Fadhilah, A. A. (2022). Uji Aktivitas Antidiabetes Ekstrak N-Heksan Jahe Merah (Zingiber officinale var rubrum) pada Tikus Galur Wistar yang Diinduksi Aloksan (Doctoral dissertation, Universitas Muhammadiyah Surakarta). Journal of Pharmacy, 2(1), 113.
Setiadi, E., Peniati, E., & Susanti, RSR (2020). Pengaruh Ekstrak Kulit Lidah Buaya Terhadap Kadar Gula Darah Dan Gambaran Histopatologi Pankreas Tikus Yang Diinduksi Aloksan. Ilmu Kehidupan , 9 (2), 182-183.
Tandi, J., Wulandari, A., & Asrifa, A. (2017). Efek ekstrak etanol daun gendola merah (Basella alba L.) terhadap kadar kreatinin, ureum dan deskripsi histologis tubulus ginjal tikus putih jantan (Rattus norvegicus) diabetes yang diinduksi streptozotocin. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy)(e-Journal), 3(2), 94-101.
Tandra, H. (2014). Strategi mengalahkan komplikasi diabetes dari kepala sampai kaki. Gramedia Pustaka Utama.
Vanesa, A., Riga, R., & Ikhsan, M. H. (2023). Aktivitas Antioksidan Jamur Endofitik Rs-1 Dari Andrographis Paniculata (Sambiloto) Menggunakan Media Beras Merah. Spin Jurnal Kimia & Pendidikan Kimia, 5(1), 107.
Worotikan, R. V., Tuju, E. A., & Kawuwung, F. (2018). Analisis efektivitas antidiabetes ekstrak etanol buah andaliman (Zanthoxylum acanthopodium DC) pada histopatologi ginjal tikus putih (Rattus novergicus) yang diinduksi alloksan. JSME (Jurnal Sains, Matematika & Edukasi), 5(1), 30.
Yuziani, Harefa, A. T, & Khairunnnisa, Z. (2023). Uji Efek Nefroprotektif Ekstrak Etanol Daun Kari (Murraya Koenigii (L.) Spreng) Terhadap Kadar Blood Urea Nitrogen (BUN) Dan Kadar Kreatinin Tikus Putih Jantan Galur Wistar (Rattus novergicus) Yang Diinduksi Doksorubin. Jurnal Riset Rumpun Ilmu Kesehatan (JURRIKES), 2(2), 121-122.
Copyright (c) 2024 Nurul Maurito Fadillah, Husnarika Febriani, Syukriah syukriah
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
