Morphological Response of Local Cowpea Varieties of Southwest Maluku to Drought Stress with PEG 6000 Induction in the Germination Phase
Abstract
Cowpea (Vigna unguiculata L. Walp), commonly known as kacang merah, is a type of legume native to southwest Maluku. This area is one of the areas with limited water availability, which causes stress to the plants. Drought stress can inhibit plant morphological growth starting from germination phase to reproductive phase. Simulation of drought stress environment can use PEG 6000. This study aims to analyze the morphological response of drought stress treatment with PEG 6000 induction in the germination phase of local cowpea varieties in Southwest Maluku. The study used a completely randomized design with PEG 6000 concentration treatment on different local varieties. The research stages consisted of preparation of PEG 6000 solution according to treatment concentration, seed germination for 7 days, and measurement of germination parameters (number of seventh day sprouts, final germination percentage, root and shoot length of sprouts, wet and dry weight of sprouts). The data obtained were analyzed using ANOVA and then Duncan's test. The results showed that PEG 6000 induction affected the morphological response of each local cowpea variety in Southwest Maluku at the germination stage. Variety KM6 showed better morphological response than other varieties in all PEG 6000 concentration treatments.
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Adi, H. P. (2011). Kondisi dan konsep penanggulangan bencana kekeringan di Jawa Tengah. Prosiding Seminar Nasional Mitigasi dan Ketahanan Bencana. Universitas Islam Sultan Agung, Semarang, 26 Juli 2011
Ali, A.S., & Elozeiri, A.A. (2017). Metabolic Process During Seed Germination. In: Advanced in Seed Biology. Lopez, J.C.J (Ed), Intech: Croatia. http://dx.doi.org/10.5772/intechopen.70653
Basal, O., Szabo, A., & Veres, S. (2020). PEG-induced drought stress effects on soybean germination parameters. Journal of Plant Nutrition, 43(12), 1768 - 1779, https://doi.org/10.1080/01904167.2020.1750638
Bhattacharya, A. (2021). Effect of Soil Water Deficit on Growth and Development of Plants: A Review. In: Soil Water Deficit and Physiological Issues in Plant. Springer: Singapore. https://doi.org/10.1007/978-981-33-6276-5_5
Carvalho, M., Castro, I., Moutinho-Pereira, J., Correia, C., Egea-Cortines, M., Matos, M., Rosa, E., Carnide, V., & Lino-Neto, T., (2019). Evaluating stress responses in cowpea under drought stress. J. Plant Physiol, 241, 153001. https://doi.org/10.1016/j.jplph.2019.153001
Dietz, K.J., Zorb, C., Geilfus, C.M. (2021). Drought and crop yield. Plant Biology, 23(6), 881–893. https://doi.org/10.1111/plb.13304
D’souza, A.A., & Shegokar, R. (2016). Polyethylene glycol (PEG): A versatile polymer for pharmaceutical applications. Expert Opin. Drug Deliv 13, 1257–1275. https://doi.org/10.1080/17425247.2016.1182485
El-Maarouf-Bouteau, H. (2022). The seed and the metabolism regulation. Biology, 11(168), 1-16. https://doi.org/10.3390/biology11020168
Efendi, R. (2009). Metode dan karakter seleksi soleransi genotipe jagung terhadap cekaman kekeringan. Tesis. Institut Pertanian Bogor.
Faidah, U. (2013). Pengaruh invigorasi menggunakan polietilena glikol (PEG) terhadap viabilitas benih kacang hijau (Vigna radiata varietas Kutilang). Skripsi, Universitas Islam Negeri.
Himaja, R., Radhika, K., Bayyapu, R.K., & Raghavendra, M. (2023). Screening of chickpea (Cicer arietinum L.) genotypes for germination and early seedling growth under peg 6000 induced drought stress. Legume Research - An International Journal, 46(7), 813-821. 10.18805/LR-4183
Hussain, S., Hussain, S., Qadir, T., Khaliq, A., Shraf, U.,Parveen, A., Saqib, M., & Rafiq, M. (2019) Drought stress in plants: an overview on implications, toler-ance mechanisms and agronomic mitigation strate-gies. Plant Science Today, 6(4), 389–402. https://doi.org/10.14719/pst.2019.6.4.578
Indraswati, D. S., Zulkifli, & Handayani, T.T. (2015). Uji ketahanan pada kecambah padi gogo (Oryza sativa L.) terhadap cekaman kekeringan yang diinduksi oleh polietilen glikol 6000. Prosiding Seminar Nasional Swasembada Pangan, Politeknik Negeri Lampung, 16–2
Jiang, W., & Lafitte, R. (2007). Ascertain the effect of PEG and exogenous ABA on rice growth at germination stage and their contribution to selecting drought tolerant genotypes. Asia J. Plant Sci, 6, 684-687.
Kaya, M. E., & Rehatta, H. (2013). Pengaruh perlakuan pencelupan dan perendaman terhadap perkecambahan benih sengon (Paraserianthes falcataria L.). Agrologia, 2(1), 10-16.
Karuwal, R. L., Suharsono, Tjahjoleksono, A., & Hanif, N. (2021). Short communication: characterization and nutrient analysis of seed of lokal cowpea (Vigna unguiculata) varieties from Southwest Maluku, Indonesia. Biodiversitas, 22(1), 85–91. https://doi.org/10.13057/biodiv/d220112
Mahpara, S., Zainab, A., Ullah, R., Kausar, S., Bilal, M., Latif, M.I., Arif, M., Akhtar, I., Al-Hashimi, A., Elshikh, M.S., Zivcak, M., & Zuan, A.T.K. (2022). The impact of PEG-induced drought stress on seed germination and seedling growth of dierent bread wheat (Triticum aestivum L.) genotypes. PLOS ONE, 17(9), e0274206. https://doi.org/10.1371/journal.pone.0274206
Nio, S. A., Tondais, S. M., & ButarButar, R. (2010). Evaluasi indikator toleransi cekaman kekeringan pada fase perkecambahan padi (Oryza sativa). Jurnal Biologi, 14(1), 50-54.
Pejić, B., Mačkić, K., Mikić, A., Cupina, B., Peksen, E., Krstic, D., & Antanasovic, S. (2013). Effect of water stress on the yield of cowpea (Vigna unguiculata L. Walp.) in temperate climatic conditions. Contemp Agric, 62, 168-176.
Purcell, L. C., Salmeron, M., & Ashlock, L. (2014). Soybean Growth and Development. Arkansas Soybean production Handbook.
Rosawanti, P. (2015). Respon pertumbuhan kedelai (Glycine max (L) Merr.) terhadap cekaman kekeringan. Jurnal Ilmiah Pertanian dan Kehutanan, 2(1), 35-44.
Siaga, E., Maharijaya, A., & Rahayu, M.S. (2016). Plant growth of eggplant (Solanum melongena L.) in vitro in drought stress polyethylene glycol (PEG). Biovalentia Biol. Res. J, 2(1), 10–17.
Singh, A.K., Srivastava, J.P., Lal, J.P. (2016). Effect of PEG-6000 induced osmotic stress on germination, growth and nutrient uptake of two lentil [Lens culinaris (Medikus)] genotypes. Journal of Food Legumes, 29(3), 188-194.
Susanto, A.N., & Sirappa, M.P. (2005). Prospects and development strategy of corn to support food security in Maluku. Jurnal Litbang Pertanian, 24(2), 70-79.
Verslues, P.E., Agarwal, M., Agarwal, K.S., & Zhu, J. (2006). Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. The Plant Journal, 45, 523–539. https://doi.org/10.1111/j.1365-313X.2005.02593.x
Zahra, N., Wahid, A., Hafeez, M.B., Ullah, A., Siddique, K.H.M., & Farooq, M. (2021). Grain development in wheat under combined heat and drought stress: Plant responses and management. Environmental and Experimental Botany, 188(104517). https://doi.org/10.1016/j.envexpbot.2021.104517

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