Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil

Immobilization of Lipase Enzim from Rice Bran (Oryza Sativa L.) on Activated Carbon: Characterization and Test the Stability

  • Firdaus Firdaus Department Of Chemistry, Faculty Mathematics and Natural Science, Hasanuddin University
  • Seniwati Dali Department Of Chemistry, Faculty Mathematics and Natural Science, Hasanuddin University
  • Hendra J. Rusman Department Of Chemistry, Faculty Mathematics and Natural, Hasanuddin University
DOI: https://doi.org/10.30598//ijcr.2017.5-fir
Keywords: Rice bran, lipase of enzymes, immobilization of enzymes, activated carbon, characterization, stability of the enzyme.

Abstract

This research aims to immobilization; characterize the enzyme of immobilized, test the effectiveness of the enzyme of immobilized. This research begins with the immobilization to process of enzyme lipase using activated carbon matrix, enzyme characterization covering of immobile determination of temperature and pH optimum of the enzyme of immobilized, as well as test the stability of work covering immobilized of enzyme the test thermal stability and repeated use. The results showed that the immobile of enzyme work optimally at 50oC of temperature and pH 6.5 with each activity 0.040 U/mL; research results also showed that the immobile of enzyme has higher thermal stability in comparison with the free enzyme: with the relative activity of 57.50% at the time of 45 minutes of exposure and the exposure time at 47.50% at 75-105 minutes and it can be used as many as six times with the relative activity of 52.5% in 6 times of use.

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Published
2017-07-31
How to Cite
(1)
Firdaus, F.; Dali, S.; Rusman, H. Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, Dan Uji Stabilitas Kerja Enzim Imobil. Indo. J. Chem. Res. 2017, 5, 32-36.
Issue
Vol 5 No 1 (2017): Edisi Bulan Juli (Edition For July)
Section
Research articles

Copyright (c) 2017 Firdaus, Seniwati Dali, Hendra J. Rusman

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