Extraction of The Chemical Components of Dengen Leaves (Dillenia serrata Thunb) by MAE Method and Activity Test as Antioxidant and Toxicity

  • Nasriadi Dali Wela Department of Chemistry, Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kampus Hijau Bumi Tridharma Anduonohu, Kendari 93232
  • Seniwati Dali Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan Km.10 Tamalanrea, Makassar 90245
  • Armadi Chairunnas Department of Biology, Faculty of Mathematics and Natural Sciences, University of Nahdlatul 'Ulama Sultra Jl. Mayjen Katamso Lr. Satya Kencana, Kendari 93116
  • Hilda Ayu Melvi Amalia Study Program of Tadris Biology, Faculty of Tarbiyah and Teacher Training, Institut Agama Islam Negeri, Jl. Sultan Qaimuddin No. 17, Kendari 93563
  • Sri Ayu Andini Puspitasari Department of Public Health, Faculty of Public Health, Halu Oleo University, Kampus Hijau Bumi Tridharma Anduonohu, Kendari 93232
Keywords: Antioxidant, Dillenia serrata, Microwave, Extraction, Toxicity.

Abstract

Research on the extraction of chemical components of Dengen (Dillenia serrata Thumb) leaves using the MAE (microwave-assisted extraction) method and activity as an antioxidant and toxicity test has been carried out. This study aimed to extract the chemical components of Dengen leaves using the MAE method and to test the antioxidant activity and toxicity of the ethanol extract of Dengen leaves. The chemical components of Dengen leaves were extracted by the MAE method and obtained ethanol extract with a yield of 47%. Dengen leaves ethanol extract was partitioned with n-hexane and ethanol as solvents and obtained yields of 5% (n-hexane) and 65% (ethanol). The chemical components of Dengen leave ethanol extract were identified by phytochemical screening. The results of phytochemical screening showed the presence of secondary metabolites of alkaloids, flavonoids, saponins, polyphenols, terpenoids, and steroids. The antioxidant activity test of the ethanol extract of Dengen leaves was carried out using the DPPH (2,2-diphenyl-1-picrihydrazil) method and obtained the value of IC50 = 100,363 ppm (strong antioxidant). A toxicity test of the ethanol extract of Dengen leaves was carried out using the BSLT (Brine Shrimp Lethality Test) method and obtained the value of LC50 = 18.3443 ppm (very toxic).

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Published
2022-09-29
How to Cite
(1)
Wela, N.; Dali, S.; Chairunnas, A.; Amalia, H.; Puspitasari, S. A. A. Extraction of The Chemical Components of Dengen Leaves (Dillenia Serrata Thunb) by MAE Method and Activity Test As Antioxidant and Toxicity. Indo. J. Chem. Res. 2022, 10, 74-82.
Section
Research articles