PENGARUH INHALASI MINYAK KAYU PUTIH (Melaleuca leucadendron) TERHADAP GAMBARAN HISTOLOGI LAMBUNG MENCIT JANTAN (Mus musculus) YANG DIINDUKSI STRES AKUT

  • Brigita Elsa Kabrahanubun Fakultas Kedokteran Universitas Pattimura
  • Halidah Rahawarin
  • Yuniasih M.J Taihuttu
  • Juliet Sinanu
  • Indrawati Kushadiani
Keywords: inhalation, cajuput oil, gastric histology, stress, mice

Abstract

Damage on gastric mucosa is caused by an imbalance of defensive factors and aggressive factors. This can be due to various factors, one of which is stress. Cajuput oil as aromatherapy is proven to prevent stress because it contains 1,8 cineole, α-terpineol, and 4-terpineol which have a sedative-hypnotic effect. This study aims to determine the effect of inhalation of cajuput oil on the gastric histology of mice. This research is a true experimental with a post test only control group design. The research subjects were 16 adult male mice taken by simple random sampling and divided into normal group (KN), negative control group (K-), positive control group (K+), and cajuput oil inhalation group (P). Groups K+, K-, and P were given stress treatment with the Tail Suspension Test (TST) method for six minutes for 14 day. Surgery was perfomed on the 15th day and histology preparations were made. The average score of gastric mucosal damage in cajuput oil group was lower when compared to the stress group although the results of the Tukey test showed no significant results between these two group. It can be concluded that cajuput oil with an inhalation dose 0.5 mL has anti-stress effect that prevent gastric mucosal damage in mice which acute stress induced.

Downloads

Download data is not yet available.

References

Liu JJW, Vickers K, Reed M, Hadad M. Re-conceptualizing stress: Shifting views on the consequences of stress and its effects on stress reactivity. PLoS One. 2017;12:1–14.

Musradinur. Stres dan cara mengatasinya dalam perspektif psikologi. J Edukasi. 2016;2:183–200.

Fransisca M, Chris A. Hubungan tingkat stres pada kelompok individu yang bernyanyi rutin dan tidak bernyanyi. Tarumanagara Med J. 2020;3:150–5.

Brosschot JF, Verkuil B, Thayer J. Generalized unsafety theory of Stress : Unsafe environments and conditions , and the default stress response. Int J Environ Res Public Health. 2018;15:1–27.

Paulmann S, Furnes D, Bøkenes AM, Cozzolino PJ. How psychological stress affects emotional prosody. PLoS One. 2016;11:1–21.

Nurmina, Netrawati, Rinaldi. Pelatihan manajemen stres dan relaksasi bagi ibu rumah angga terdampak covid 19 di Kelurahan pasie Nan Tigo Kota Padang. J Plakat. 2020;2:150–9.

Musabiq SA, Karimah I. Gambaran stres dan dampaknya pada mahasiswa. InSight. 2018;20:75–83.

Ennab W, Mustafa S, Wei Q, Lv Z, Kavita NMX, Ullah S. Resveratrol protects against restraint stress effects on stomach and spleen in adult male mice. Animals. 2019;9:1–18.

Huerta-franco MR, Vargas-luna M, Montes-frausto JB, Morales-mata I, Ramirez-padilla L, Huerta-franco MR, et al. Effect of psychological stress on gastric motility assessed by electrical bio-impedance. 2012;18:5027–33.

Elsaed WM, Alahmadi AM, Al-Ahmadi BT, Taha JA, Tarabishi RM. Gastroprotective and antioxidant effects of fluvoxamine on stress-induced peptic ulcer in rats. J Taibah Univ Med Sci [Internet]. 2018;13:1–10. Available from: https://doi.org/10.1016/j.jtumed.2018.04.010

Bunaya R, Romus I, Siregar FM, Juananda D. The effect of immobilization stress on gastric mucosal histopathology in white mice (Mus musculus) male swiss webster strain. Mol Cell Biomed Sci [Internet]. 2020;4:34. Available from: https://cellbiopharm.com/ojs/index.php/MCBS/article/view/77

Kartika D, Lindawaty P, Sewu S. Pelayanan kesehatan tradisional dan perlindungan hukum bagi pasien. J Huk Kesehat. 2016;2:1–16.

Indonesia KT. Pengembangan kesehatan tradisional Indonesia : Konsep , strategi dan tantangan. J Penelit dan Pengemb Pelayanan Kesehat. 2017;1:17–31.

Siddique S, Parveen Z, Mazhar S. Chemical composition , antibacterial and antioxidant activities of essential oils from leaves of three melaleuca species of Pakistani flora. Arab J Chem [Internet]. 2020;13:67–74. Available from: https://doi.org/10.1016/j.arabjc.2017.01.018

Zhang X, Guo Y, Guo L, Jiang H, Ji Q. In vitro evaluation of antioxidant and Antimicrobial activities of melaleuca alternifolia essential Oil. 2018;2018.

Thi T, My A, Phuong T, Thanh T, Hieu T. Evaluation of the Inhibitory Activities of COVID-19 of Melaleuca cajuputi Oil Using Docking Simulation. 2020;5:6312–20.

Korona-Glowniak I, Glowniak-Lipa A, Ludwiczuk A, Baj T, Malm A. The in vitro activity of essential oils against helicobacter pylori growth and urease activity. Molecules. 2020;25.

Activitie A, Pujiarti R, Ohtani Y, Ichiura H. Chemical compositions , antioxidant and antifungal activities of melaleuca leucadendron linn . Leaf oils from Indonesia. 2012;3:23–7.

Patramurti C, Amin R, Nastiti CMRR, Hariono M. A review on the potencyof melaleuca leucadendron leaves solid waste in wood preservation and its in silico prediction upon biological activities. 2020;

Kim KY, Seo HJ, Min SS, Park M, Seol GH. The effect of 1,8-cineole inhalation on preoperative anxiety : A randomized clinical trial. 2014;

Theodorus E, Muhartono M, Putri GT. Pengaruh pemberian ekstrak etanol rimpang lengkuas (Alpinia galanga) terhadap gambaran histopatologi otak mencit (Mus musculus L) yang diinduksimonosodium glutamate. JIMKI J Ilm Mhs Kedokt Indones. 2020;7:14–20.

Wiradi A, Fidiawati WA, Munir SM. Gambaran histopatologi paru mencit (Mus musculus) setelah pemaparan paraquat. J Ilmu Kedokt. 2018;11:7.

Pratiwi R, Posangi J, Fatimawali. Ujiefek analgesik ekstrak etanol daun gedi (Abelmoschus manihot (L.) Medik) pada mencit (Mus musculus). J e-Biomedik. 2013;1:571–80.

Aslam M. Tail suspension test to evaluate the antidepressant activity of experimental drugs. 2016;292–4. 25. Jahra W,Muhartono, Windi R. Pengaruh pemberian minuman ringan berkabonasi terhadap gambaran histopatologi esofagus tikus putih (Rattus novergicus) jantan galur Sprague dawley. Medula. 2019;8:193–9.

Damayanti N, Hadiati T. Pengaruh pemberian aromaterapi terhadap tingkat insomnia lansia. Diponegoro Med J. 2019;8:1210–6.

Sembor P, Lintong P, Kairupan C. Gambaran histopatologik mukosa lambung tikus putih (Rattus norvegicus) yang diinduksi kebisingan dan diberikan ranitidin. e-Biomedik (eBm). 2013;1:966–71.

Tussakinah W, Masrul M, Burhan IR. Hubungan pola Makan dan tingkat stres terhadap kekambuhan gastritis di wilayah kerja Puskesmas Tarok Kota Payakumbuh tahun 2017. J Kesehat Andalas. 2018;7:217.

Widya Murni A. Kadar kortisol plasma pada dispepsia fungsional dengan gangguan psikosomatik. J Penyakit Dalam Indones. 2020;7:15.

Sibernagl S, Lang F. Teks & atlas berwarna patofisiologi. 3rd ed. Iskandar M, Agustina L, Agustin S, editors. Jakarta: EGC; 2017. 156–7 p.

Takeuchi K, Amagase K. Roles of cyclooxygenase, prostaglandin E2 and EP receptors in mucosal protection and ulcer healing in the gastrointestinal tract. Curr Pharm Des. 2018;24:2002–11.

Nur Azlina MF, Kamisah Y, Chua KH, Qodriyah HMS. Tocotrienol attenuates stress-induced gastric lesions via activation of prostaglandin and upregulation of COX-1 mRNA. Evidence-based Complement Altern Med. 2013;

Magierowski M, Jasnos K, Kwiecien S, Drozdowicz D, Surmiak M, Strzalka M, et al. Endogenous prostaglandins and afferent sensory nerves in gastroprotective effect of hydrogen sulfide against stress-induced gastric lesions. PLoS One. 2015;10:1–21.

Hemanth G V., Kudagi BL, Buchineni M, Pathapati RM, Rayam S. Anti-stress activity of ocimum sanctum and alprazolam in animal models. Int J Basic Clin Pharmacol. 2019;8:2224–7.

Katzung B, Masters S, Trevor A. Basic & clinical pharmacology. 12th ed. Vol. 12, Annual Reports in Medicinal Chemistry. San Francisco; 1977. 378 p.

Manouchehri N, Abbasi-Maleki S, Mousavi Z. A comparison of the anxiolytic and hypnotic effects of origanum majorana essential oil and alprazolam in male mice. Herb Med J. 2019;4:18–26.

Dougnon G, Ito M. Inhalation administration of the bicyclic ethers 1,8-And 1,4-cineole prevent anxiety and depressive-like behaviours in mice. Molecules. 2020;25:1–13.

Nila G, Kristiningrum W, Afriyani LD. Efektivitas aromaterapi lavender untuk mengurangi kecemasan menghadapi persalinan pada ibu hamil trimester III di wilayah kerja Puskesmas Bergas. J Holistics Heal Sci. 2019;1:104–5.

Rocha Caldas GF, Oliveira ARDS, Araújo AV, Lafayette SSL, Albuquerque GS, Silva-Neto JDC, et al. Gastroprotective mechanisms of the monoterpene 1,8-cineole (eucalyptol). PLoS One. 2015;10:1–17.

Published
2022-11-18
How to Cite
1.
Kabrahanubun B, Rahawarin H, Taihuttu Y, Sinanu J, Kushadiani I. PENGARUH INHALASI MINYAK KAYU PUTIH (Melaleuca leucadendron) TERHADAP GAMBARAN HISTOLOGI LAMBUNG MENCIT JANTAN (Mus musculus) YANG DIINDUKSI STRES AKUT. PAMERI [Internet]. 18Nov.2022 [cited 14Jun.2024];4(2):65-3. Available from: https://ojs3.unpatti.ac.id/index.php/pameri/article/view/7618
Section
Articles