PAPARAN EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP GAMBARAN HISTOPATOLOGI HATI MENCIT (Mus musculus)
Keywords:
Sea Cucumber, Potassium Bromate (KBrO3), Histopathology
Abstract
Background: In Indonesia, traditionally sea cucumber (Holothuria scabra) is often used as a cure gout. The purpose of the research is to determine the effects of sea cucumber (Holothuria scabra) extracts against liver histopathology picture mice (Mus musculus).
Method: Mice used were male mice as much as 15 tails and were divided into 5 groups. Each group consisted of three mice. Group I as a positive control given allopurino l0.1%. The second group was given distilled water as a negative control. Group III, IV and V as the treatment group was given sea cucumber extract with each concentration of 5%, a concentration of 10% and 15% concentration.
Result: The results were analyzed quantitatively, showed that the administration of potassium bromate showed the presence of fatty and necrosis. But after being given a sea cucumber extract with a concentration of 10% and a concentration of 15% still showed the presence of inflammatory cells and remnants of fatty liver (same as the picture of liver histopathology in the positive control). At a concentration of 5% normal liver histopathology same as the picture of liver histopathology in the negative control.
Conclution: Sea cucumbers with a concentration of 5% can be used as a gout medicine without any side effects for conditions onal liver function.
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References
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Ridzwan. 2005. Gout dan Hiperurisemia. Devisi Reumatologi Departemen Ilmu Penyakit Dalam. Fakultas Kedokteran Universitas Indonesia RSUPNCM. Jakarta Vol. 22.
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Sies H., Stahl W., 1995. Vitamins E and C, β-carotene, and other carotenoids as antioxidants 1-3 .American Journal Clinical Nutrition 62(suppl):1315S-21S.
Svetashev V.I., Levin V. S., Lam C. N., Nga D. T., 2002. ipid and fatty acid composition of holothurians from tropical and temperate waters (abstract). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. Volume 98. Issue 4:89-494.
Tovar A.M.F., Mourão P.A.S., 1991. High affinity of a fucosylated chondroitin sulfate for plasma low density lipoprotein (abstract). Atherosclerosis. Volume 126. Issue 2:185-195.
Trubus, 2006. Reportase Malaysia, Obat Mujarab dari Laut. Trubus edisi Minggu 02 Juli dalam Cara Sehat dengan Teripang/Sea Cucumber.
Wulf, D. 2002. Free radical in the physiological control of cell function. Physiol. Rev. 82: 47-95.
Yuda, A. 2007. Bioaktivitas senyawa dimer dari isoeugenol dengan enzim peroksidase dari tanaman sawi. Skripsi Sarjana S1 Departemen Kimia, FMIPA, UI. Disertasi diterbitkan. Depok.
Ahmad, U., & Sharafatullah, T. 2008. Hepato-protective natural compounds. Pak J Pharmacol. 25 (02):59-68.
Amalina, N. 2009. Uji Toksisitas Akut Ekstrak Valerian (Valeriana Officinalis) Terhadap Hepar Mencit Balb/C. Karya Tulis Ilmiah. Semarang: Fakultas Kedokteran Universitas Diponegoro.
Farouk A.E., Ghouse F.A.H., Ridzwan B.H, 2007. New Bacterial Species Isolated from Malaysian Sea Cucumbers with Optimized Secreted Antibacterial Activity. American Journal of Biochemistry and Biotechnology 3 (2): 60-65.
Fredelina B.D., Ridzwan B.H, Zainal Abidin A.A., Kaswandi M.A., Zaiton H., et al,. 1999. Fatty acid compositions in local sea cucumber, Stichopus chloronatus for wound healin (abstrack). General Pharmacology. Volume 33. Issue 4:337-340.
Guyton dan Hall. 2006. Informasi Seputar Dunia Hewan, (Online), (www.blogspot.tikus-putih.com), diakses 12 Maret 2016.
Ha B.J., Lee J.Y., 2003. The effect of chondroitin sulfate against CCl4-induced hepatotoxicity (Abstrac). Biol Pharm Bull. Vol 26; No 5.
Hasan, H. 2013. Efek Antiurisemia Ekstrak Teripang Pasir (Holothuria scabra) pada Kelinci Jantan (Oryctolagos cuniculus). Jurusan Farmasi, Universitas Negeri Gorontalo.
Himawan, S. 1992. Patologi. Jakarta: Penerbit Buku Kedokteran EGC. hlm. 86.
Jing, Y.X., Yuan , Y.S., Jin, S.C., Shu, X.L., Ruili, L., Wen, X.L., Guo, T.X., & Qing, N.L. 2010. Protective effects of fullerenol on carbon tetrachloride-induced acute hepatotoxicity and nephrotoxicity in rats. Carbon. 48:1388-196.
Kariya Y., S. Watabe, K. Hashimoto, K. Yoshida, 1990. Occurrence of Chondroitin Sulfate E in Glycosaminoglycan Islated from the Body Wall of Sea Cucumber Stichopus japonicus. The Journal of Biological Chemistry. Vol. 265. No. 9. Issue of March 25:5081-5085.
Kasai T., 2003. Lemak Contents and fatty acid compotition of total lemak of sea cucumber Stichopus japonicus and Konowata (salted sea cucumber entrails). Food Sci. Technol. Res., 9 (1):45-48.
Khadr, M.N., M ahdy, K.A., El-Shamy, K.A. Morsy, F.A., El-Zayat, S.R., & Abd-Allah, A.A. 2007. Antioxidant activity and hepatoprotective potential of black seed, honey and sylimarin on experimental liver injuries induced by CCL4 in rats. J Appl Sci 24: 3909-3917.
Klaassen, C.D. & Watkins III, J.B. 1999. Cassaret & Doull’s Toxicology: The basic science of poisons. McGraw-Hill Companies, Inc., USA: vii + 861 hlm.
Long, R. G., & Scott, B. B. 2005. Specialist training in gastroenterology and liver disease. Elsevier Mosby, Philadelphia: xxvii + 1786 hlm.
Lu, F. C. 1995. Toksikologi dasar: Asas, organ sasaran, dan penilaian resiko.Terj dari Basic Toxicology: Fundamentals, target organs, and risk assesment oleh Nugroho, E. Penerbit Universitas Indonesia (UI-Press), Jakarta: xv + 429 hlm.
Madhavan R., 1998. Mengesankan Kehadiran Vitamin E dan Unsur-unsur Logam dalam tiga spesies timun laut. Tesis Sarjana Muda Sains Bioperubatan (Kepujian). Fakulti Sains Kesihatan Bersekutu, Universitas Kebangsaan Malaysia, dalam Hawa I, Zulaikah M, Jamaluddin M, Zainal Abidin AA, Kaswandi MA, Ridzwan BH, 1999. The Potential of the Coelomic Fluid in sea cucumber as an Antioxidant. Mal J Nutr 5:55-59.
Matsuno T., Tsushima M., 2000. Comparative biochemical studies of carotenoids in sea cucumbers*1(abstract). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. Volume 111. Issue 4: 597-605.
Maurao P.A.S., Pereira, M.S., Pavao, M.S.G., Mulloy, B., Tollefsen, D.M, et al., 1996. Structure and Anticoagulant Activity of a Fucosilated Chondroitin Sulfate fron Echinoderm. The Journal of Biological Chemistry, Vol. 271, No. 39, Issue of September 27:23973-23984.
Nation Master. 2012. Alcoholic liver diseases statistics, (Online), (http://www.nationmaster.com/graph/mor_alc_liv_dis-mortality-alcoholic-liver-disease), diakses 27 Februari 2016.
Omi, W. 2016. Efektifitas Ekstrak Teripang Pasir (Holothuria scabra) Sebagai Antiurisemia Untuk Menurunkan Kadar Asam Urat Pada Mencit (Mus muculus). Skripsi tidak dipublikasikan. Ambon: FKIP Pendidikan Biologi, Universitas Pattimura.
Ridzwan. 2005. Gout dan Hiperurisemia. Devisi Reumatologi Departemen Ilmu Penyakit Dalam. Fakultas Kedokteran Universitas Indonesia RSUPNCM. Jakarta Vol. 22.
Rhodes, R.A., & Tanner, G.A. 1995. Medical physiology. 1st ed. Little, Brown and Company, USA: x + 839 hlm.
Robbins, S. L. dan Kumar, V. 1992. Buku Ajar Patologi 1. Surabaya: Penerbit Buku Kedokteran EGC. hlm. 14-17.
Rustam, 2006, Budidaya Teripang, Pelatihan Budidaya Laut (Coremap Fase II Kab. Selayar), Yayasan Mattirotasi, Makassar.
Sies H., Stahl W., 1995. Vitamins E and C, β-carotene, and other carotenoids as antioxidants 1-3 .American Journal Clinical Nutrition 62(suppl):1315S-21S.
Svetashev V.I., Levin V. S., Lam C. N., Nga D. T., 2002. ipid and fatty acid composition of holothurians from tropical and temperate waters (abstract). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. Volume 98. Issue 4:89-494.
Tovar A.M.F., Mourão P.A.S., 1991. High affinity of a fucosylated chondroitin sulfate for plasma low density lipoprotein (abstract). Atherosclerosis. Volume 126. Issue 2:185-195.
Trubus, 2006. Reportase Malaysia, Obat Mujarab dari Laut. Trubus edisi Minggu 02 Juli dalam Cara Sehat dengan Teripang/Sea Cucumber.
Wulf, D. 2002. Free radical in the physiological control of cell function. Physiol. Rev. 82: 47-95.
Yuda, A. 2007. Bioaktivitas senyawa dimer dari isoeugenol dengan enzim peroksidase dari tanaman sawi. Skripsi Sarjana S1 Departemen Kimia, FMIPA, UI. Disertasi diterbitkan. Depok.
Published
2016-03-15
How to Cite
Matruty, Y., & Watuguly, T. (2016). PAPARAN EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP GAMBARAN HISTOPATOLOGI HATI MENCIT (Mus musculus). BIOPENDIX: Jurnal Biologi, Pendidikan Dan Terapan, 2(2), 160-169. https://doi.org/10.30598/biopendixvol2issue2page160-169
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Articles
