Produksi Oligomer Kitosan dari Limbah Udang Windu (Panaeus monodon) Menggunakan Enzim Kitosanase dari Isolat Bakteri Klebsiella sp.

Chitosan Oligomer Production From Waste Tiger Shrimp (Penaeus monodon) Using Enzymes Chitosanase of Bacterial Isolates Klebsiella sp

  • Sarni Sarni Midwifery Academic of National Healting Fondation, Jl. Palagimata, Baubau-Indonesia
  • Hasnah Natsir Department of Chemistry, Faculty of Mathematics and Natural Science, University of Hassanuddin-Indonesia
  • Seniwati Dali Department of Chemistry, Faculty of Mathematics and Natural Science, University of Hassanuddin-Indonesia
Keywords: Waste Tiger Shrimp,chitosan, chitosanase,chitosan oligomer.

Abstract

Chitosan is a biopolymer that is the main content of D-glucosamine and several parts of N-acetyl-D-glucosamine bound to β- (1-4) glucoside. Chitosan receive special attention as functional biopolymers for applications in various fields. Chitosan is more effectively absorbed into the human body when it gets converted into chitosan oligomer form. Chitosan oligomer is a mixture of oligomers of D-glucosamine  are formed through a process of severing ties depolymerization of chitosan with β-glycosidic. This study aims to produce chitosan oligomer of waste tiger shrimp (Penaeus monodon) using enzyme kitosanase of bacteria Klebsiella sp. Chitosan oligomer produced by using the enzyme chitosanase at a temperature of 40 °C and pH 8 with the activity of 0.309 U/mL (5,235 U/mg) obtained in the form of a mixture of monomer to octamer, which  soluble in acetic acid 0.25% to 0.5%, having intrinsic viscosity decreases with increasing time of incubation is 0.195 (1 hour incubation); 0.9 (incubation 2 hours) and 0.7 (incubation 3 hours) with molecular weight range of 4103.12 g/mol (incubation 1 hour) ; 1483.48 g/mol (incubation 2 hours) and 1065.79 g/mol (incubation 3 hours).

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Published
2016-01-31
How to Cite
(1)
Sarni, S.; Natsir, H.; Dali, S. Produksi Oligomer Kitosan Dari Limbah Udang Windu (Panaeus Monodon) Menggunakan Enzim Kitosanase Dari Isolat Bakteri Klebsiella Sp. Indo. J. Chem. Res. 2016, 3, 283-289.
Issue
Vol 3 No 2 (2016): Edisi Bulan Januari (Edition For January)
Section
Short communication

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