Toksisitas Oligomer Kitosan Derajat Deasetilasi Rendah Enzimatis Menggunakan Metode Brine Srimp Lethality Test (BSLT)

Toxicity Of Chitosan Oligomer Enzymatic Low Degree Of Deacetylation Using Brine Srimp Lethality Test (Bslt) Method

  • Sarni Sarni Midwifery Academic of National Healting Fondation, Jl. Palagimata, Baubau-Indonesia
DOI: https://doi.org/10.30598//ijcr.2017.4-sar
Keywords: Chitosan oligomers, toxicity, BSLT

Abstract

Chitosan oligomers or chitooligomer is a mixture of oligomers of D-glucosamine which are formed through a process of depolymerization of chitosan with cuting its β-glycosidic bonding. Chitosan oligomer has received much attention in various fields because of the specific biological activity 10 times greater than chitosan. Chitosan oligomer having biological activity as antibacterial, antiviral, antioxidant, anti-tumor and anti-cancer. Before it is applied to humans, the bioactive compounds to be used as pharmaceutical products must pass a preliminary test using test animals. BSLT method is often used to praskrining against bioactive compounds. This method uses shrimp larvae are widely used in searches that are toxic bioactive compounds from natural materials. This study aims to determine the toxicity of chitosan oligomers enzymatic (crude) as a preliminary test before being used as a pharmaceutical product either as anti-cancer or anti microbial with BSLT method. The results showed chitosan oligomer (crude) hydrolysis of leather tiger shrimp chitosan (DD 60%) with chitosanase enzyme for each time of incubation has strong toxicity properties with a value of 36.90 ppm LC50 (1 hour incubation); 47.43 ppm (incubation 2 hours) and 104.86 ppm (incubation 3 hours).

Downloads

Download data is not yet available.
Published
2017-01-31
How to Cite
(1)
Sarni, S. Toksisitas Oligomer Kitosan Derajat Deasetilasi Rendah Enzimatis Menggunakan Metode Brine Srimp Lethality Test (BSLT). Indo. J. Chem. Res. 2017, 4, 373-377.
Issue
Vol 4 No 2 (2017): Edisi Bulan Januari (Edition For January)
Section
Research articles

Authors who publish with this journal agree to the following terms:

  1. Copyright on any article is retained by the author(s).
  2. The author grants the journal, the right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.
  3. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
  4. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
  5. The article and any associated published material is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.