Extraction And Identification Of Sulfated Polysaccharide From Gracilaria sp.

Ekstraksi dan Identifikasi Polisakarida Bersulfat Dari Sayur Karang Gracilaria sp

  • Dominggus Malle Faculty of Agriculture,Pattimura University
  • Eirene G. Fransina Department of Chemistry, Faculty of Mathematics and Natural Science, University of Pattimura-Indonesia
  • Feky Jansen Department of Chemistry, Faculty of Mathematics and Natural Science, University of Pattimura-Indonesia
DOI: https://doi.org/10.30598/ijcr.2014.1-dom
Keywords: Extraction, Sulphated polysaccharide, Gracilaria, FTIR

Abstract

Polysaccharide from Gracilaria sp (locally known as “Sayur Karangâ€) was extracted using hot alkaline solutions (NaOH and KOH). Fractionation using DEAE-Sepharose Fast Flow column showed that the polysaccharide discharged after being eluted with 0.5 – 1.0 M NaCl. Spectroscopic analysis using FTIR showed that the polysaccharide contained hydroxyl  (3420 cm-1 (NaOH frcation) dan 3446 cm-1 (KOH frcation)), carbonyl (1642 cm-1 (NaOH frcation) and 1644 cm-1 (KOH frcation)) and sulphate (1271 cm-1 (NaOH frcation) and 1262 cm-1 (KOH frcation)) groups proving that the polysaccharide is a sulphated polysaccharide. A further study is needed to elucidate the structure of the sulphated polysaccharide as well as physiochemical properties of the polysaccharide.

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Published
2014-01-31
How to Cite
(1)
Malle, D.; Fransina, E.; Jansen, F. Extraction And Identification Of Sulfated Polysaccharide From Gracilaria Sp. Indo. J. Chem. Res. 2014, 1, 83-87.
Issue
Vol 1 No 2 (2014): Edisi Bulan Januari (Edition For January)
Section
Short communication

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