PENGARUH STRES AKUT TERHADAP KADAR GULA DARAH MENCIT (Mus Musculus) DENGAN PERLAKUAN EKSTRAK ETANOL ALGA COKELAT (Sargassum sp.)
Abstract
Stress is a non-specific response in the body to conditions or conditions that occur as a result of exposure to stressors. High stress usually has a negative impact on the body, one of which is increasing blood sugar levels. Brown algae is one of the traditional marine plants and contains secondary metabolite compounds such as alkaloids, flavonoids, and steroids which are antidepressants so that they can prevent the increase in blood sugar levels. This study aims to determine the effect of acute stress on blood sugar levels of mice by giving ethanol extract of brown algae. This research is a true experimental research design with pre test and post test control group design. 30 mice were divided into normal control (KN), negative control (K-), positive control (K +) groups, ethanol extract of brown algae with a concentration of 2.5% (P1) and 5% (P2). The study was started by measuring the initial blood sugar levels in all groups, then groups K-, K +, P1, and P2 were given stress treatment with the FST method for 6 minutes for 7 days. Group K + was given alprazolam at a dose of 0.2 ml at 1 hour before treatment, while P1 and P2 were given brown algae at 30 minutes before treatment. After the treatment, it was followed by measurement of the final blood sugar level. The data from the observations were analyzed using the dependent T-test with a confidence level of 95%. The results showed that the post-test blood sugar levels in the P1 and P2 groups experienced an increase, but the increase was not significant. The results also showed that the significance values (2-tailed) in the P1 and P2 groups were 0.772 and 0.392 (sig> 0.05), meaning that it could be concluded that there was no effect of giving ethanol extract of brown algae on the prevention of increasing blood sugar levels after acute stress.
Downloads
References
Edy Susanto M. Pengaruh stres terhadap kadar hormon progesteron pada tikus wistar postpartum. J Chem Inf Model. 2019;53(9):1689–99.
Astutik W, Kuswati E. Efektivitas pemberian jus kulit manggis terhadap kadar hormonkortisol pada mencit (mus musculus) yang mengalami stres. J Skala Husada. 2014;11(1):91–5.
Adam L, Tomayahu MB. Tingkat stres dengan kadar gula darah.J Heal Sport. 2019;1(1):1–5.
Tua N, Gaol L. Teori stres: stimulus, respons, dan transaksional.Bul Psikol UGM. 2016;24(1):1–11.
Rahmayani RD, Liza RG, Syah NA. Gambaran tingkat stres berdasarkan stressor pada mahasiswa kedokteran tahun pertama program studi profesi dokter fakultas kedokteran universitas andalas angkatan 2017. J Kesehat Andalas. 2019;8(1):103.
Epidemiologi B, Masyarakat FK. Faktor-faktor yang berhubungan dengan kadar gula darah pada penderita diabetes melitus tipe 2 di rsud k.r.m.t wongsonegoro semarang.J Kesehat Masy. 2018;6(1):200–6.
Pratiwi P, Amatiria G, Yamin M. Pengaruh stress terhadap kadar gula darah sewaktu pada pasien diabetes melitus. J Kesehat. 2015;(1 april 2014):11–6.
Anita AT. Hubungan tingkat stres dengan kadar gula darah pada pasien diabetes melitus di rsud kota madiun. Vol. 53, Journal of Chemical Information and Modeling. 2015.
Setiyorini E, Wulandari NA, Efyuwinta A. Hubungan kadar gula darah dengan tekanan darah pada lansia penderita diabetes tipe 2. J Ners dan Kebidanan (Journal Ners Midwifery). 2018;5(2):163–71
Sukadiyanto S. Stress dan cara menguranginya.J Cakrawala Pendidik. 2014;1(1):55–66.
Musradinur. Stres dan cara mengatasinya dalam perspektif psikologi. Edukasi. 2016;2(July):183–200.
Puspitasari AW, Angeline L. Analisis potensi interaksi obat golongan antidepresan pada pasien skizofrenia di rumah sakit jiwa dr. soeharto heerdjan tahun 2016 analysis of potential antidepressant drug interactions in schizophrenic patients at dr. soeharto heerdjan 2016. Pharm Sci Res. 2019;6(1):13–20
Ode I, Wasahua J. Jenis-jenis alga coklat potensial di perairanpantai Desa Hutumuri Pulau Ambon. Agrikan J Ilm Agribisnis dan Perikan. 2015;7(2)
Handayani T. Fukosantin: karotenoid berharga dari makroalga coklat. Oseana. 2018;43(3):16–28.
Hasanah A. Efek jus bawang bombay (allium cepa linn) terhadap motilitas spermatozoa mencit yang diinduksi streptozotocin (stz). 2015;92–101.
Puspadewi L, Studi P, Dokter P, Umum FK, SurakartaUM. Efek ekstrak biji pepaya (carica papaya l.) terhadap kadar glukosa darah tikus putih jantan (rattus novergicus) galur wistar yang diinduksi aloksan. 2019;
Gazali M, Nurjanah N, Zamani NP. Eksplorasi senyawa bioaktif alga cokelat sargassum sp. agardh sebagai antioksidan dari pesisir barat aceh. J Pengolah Has Perikan Indones. 2018;21(1):167
Naina Y, Wulandari R, Said T, Yuni N, Rika W, Tengku RIS. Skrining komponen bioaktif ethanol 96 % sargassum sp. sebagai antibakteri terhadap vibrio harveyi screening of bioactive components ethanol 96 % sargassum sp. as an antibacterial against vibrio harveyi. J Budid Perair. 2019;3:22–33.
Vol J, Ramadhan A, Dhafir F. Pengaruh pemberian ekstrak biji pala (myristica fragrans houtt) terhadap albumin, globulin pada tikus putih (rattus norvegicus). 2014;3:27–32.
AL LD and B. Evaluation of drug activities: pharmacometrics. Acad Press London. 1964;1(1).
Sasmita FW, Susetyarini E, Pantiwati Y. Efek ekstrak daun kembang bulan (tithonia diversifolia) terhadap kadar glukosa darah tikus wistar (rattus norvegicus) yang diinduksi alloxan. Biosfea. 2017;34(1):22–31.
Cahyaningrum PL, Made Yuliari SA, Suta IBP. Antidiabetic activity test using amla fruit (phyllanthus emblica l) extract in alloxan-induced balb/c mice.J Vocat Heal Stud. 2019;3(2):53.
Putriani D, Setyawati D, Studi P, Keperawatan I, Unimus F. Relaksasi otot progresif terhadap kadar gula darah pada pasien diabetes mellitus tipe 2. Pros Semin Nas Mhs Unimus. 2018;1:135–40.
Amri F. Farmakologi alprazolam dalam mengatasi gangguan panik. J Kedokt Syiah Kuala. 2015;12(3):187–90.
Perempuan DAN, Akan Y, Operasi M. Perbedaan tingkat kecemasan antara laki-laki dan perempuan yang akan menjalani operasi elektif post premedikasi dengan alprazolam.J Komplikasi Anestesi. 2016;3(3):15–23.
Nurung SHH. Penentuan kadar total fenolik, flavonoid, dan karotenoid ekstrak etanol kecambah kacang hijau (vigna radiata l.) menggunakan spektrofotometer uv-vis (skripsi). Skripsi. 2016;80.
Adrielly C, Ferraz A, Gonçalves R, Júnior DO, Picot L, Roberto J, et al. Fitoterapia pre-clinicalinvestigations of β -carboline alkaloids as antidepressant agents: A systematic review. Fitoterapia. 2019;137(March):104196.
Maiti S, Nazmeen A, Medda N, Patra R. Flavonoids green tea against oxidant stress and in fl ammation with related human diseases. Clin Nutr Exp. 2019;1(1):1–4.
Copyright (c) 2021 PAMERI: Pattimura Medical Review
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
