PENGARUH PEMBERIAN EKSTRAK ETANOL ALGA COKELAT (Sargassum sp.) TERHADAP HITUNG JENIS LEUKOSIT MENCIT (Mus musculus) PASCA DIINDUKSI STRES AKUT
Abstract
Glucocorticoids are stress hormones that suppress normal immune responses by blocking Th1 cells. Glucocorticoids have an impact on decreasing the body's immune system and changes in leukocyte differentiation. Sargassum sp. is one type of brown algae that lives in the sea. Sargassum sp. contains metabolite compounds that are immunomodulatory. This study aims to determine the number of types of leukocytes in mice that are induced by stress and given ethanol extract of Sargassum sp. Pure experimental research design with post test only control group design. Thirty mice were divided into five treatment groups, namely the normal control group (KN), negative control (K-), positive control (K +), ethanol extract of Sargassum sp. 35% (P1) and 70% (P2). At K-, K +, P1 and P2 were given stress treatment with the FST (Forced Swim Test) method to see immobility time in mice. The treatment was given for seven days and on the 8th day the intracardial mice were taken. Observation of leukocytes from the blood smear of mice was carried out using a microscope with 1000x magnification. Observation data were analyzed by ANOVA. The results showed that the P1 group could reduce the percentage of neutrophils and monocytes but increase the percentage of lymphocytes, while the P2 group showed a decrease in the percentage of neutrophils, an increase in the percentage of lymphocytes, but could not decrease the percentage of monocytes compared to P1. The mean percentage of eosinophils and basophils did not change significantly, both in the treatment group and in the control group. However, statistical data shows no significant change between groups. So it can be concluded that stress treatment given to mice does not have a significant effect on the average percentage of monocytes, lymphocytes, neutrophils, eosinophils and basophils after being induced by stress.
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References
Larasati R. Pengaruh stres pada kesehatan jaringan periodontal. Skala Husada. 2016;13(1):81–9.
Mushidah, Muliawati R. Perubahan jumlah leukosit akibat aktivitas fisik berat pada mencit jantan balb/c. Kesehat Masy Indones. 2019;14(1):11–4.
Wardhana M. Psikoneuroimunologi di bidang dermatologi. MDVI. 2011;38(4):175–80.
Mushidah, Nasihun T, Hussaana A. Effect of Strenuous Physical Activity on Cortisol and Leukocyte in Mice. Sains Med. 2016;7(2):59–63.
Rasyid A. Mekanisme imunodepresipascastroke. Neurona. 2019;36(3):217–25.
Titisari N, Asri K, Fauzi A, Masnur I, Kurniawan I. Kadar Hormon Kortisol dan Rasio Neutrofil/Limfosit (N/L) Satwa Lutung Jawa pada Saat di Kandang Perawatan dan Kandang Karantina di Hutan Coban Talun, Batu. TERNAK Trop J Trop Anim Prod. 2019;20(1):29–37.
Hendrajid Z. Jenis leukosit mencit (mus musculus) dengan perlakuan ekstrak etanol biji pala (myristica fragrans) pasca stres akut. Program studi pendidikan dokter fakultas kedokteran universitas pattimura. Universitas Pattimura; 2020.
Rachmani S, Mayasari D. Kombinasi Farmakoterapi dan Psikoterapi Pada Pengobatan Episode Depresif Sedang Dengan Gejala Somatis Combination Of Pharmacotherapy And Psychotherapy On The Treatment Of Moderate DepressiveEpisode With Somatic Symptoms. J medula unila. 2017;7(2):133–9.
Faizah AK, Andhiarto Y, Wijayanti N. Uji Aktivitas Minyak Ikan sebagai Antidepresan pada Depresi Kronik secara In Vivo. J Said Med. 2018;10(2):56–8.
Jasmadi RN. Saffron (Crocus sativus) Sebagai Alternatif Obat Anti Depresi Rima. J Kesehat dan Sains Terap STIKes Merangin. 2019;5(2):1–8.
Sari BL, Susanti N, Sutanto S. Skrining Fitokimia dan Aktivitas Antioksidan Fraksi Etanol Alga Merah Eucheuma spinosum. Pharm Sci Res. 2015;2(2):59–68.
Sormin RBD, Soukotta D, Saiful, Risambessy A, Ferdinandus SJ. Sifat fisiko-kimia semi refined carrageenan dari kota ambon dan kabupaten maluku tenggara barat. JPHPI. 2018;21(1):92–8.
Pakidi CS, Suswati E. Potensi dan Pemanfaatan Bahan Aktif Alga Cokelat Sargassum sp. Octopus. 2017;6(1):551–62.
Radiena MSY. Analisis kandungan gizi alga hijau silpau (Dictyosphaeria versluysii). Maj BIAM. 2018;14(1):8–13.
Satyarsa ABS. Studi Pustaka: Potensi Fucoidan dari Rumput Laut Coklat (Sargassum sp.) sebagai Inovasi Terapi pada Kanker Payudara. J Med Heal. 2019;2(3):909–19.
Arwidasari AR, Cevanti TA, Soewondo IK. Effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattusnorvegicus). Padjadjaran J Dent. 2019;31(1):73.
Altinok-Yipel F, Tekeli IO, Ozsoy SY, Guvenc M, Sayin S, Yipel M. Investigation of hepatoprotective effect of some algae species on carbon tetrachloride-induced liver injury in rats. Arch Physiol Biochem. 2019;1(1):1–5.
Faricha F, Firdaus M. The effect of phlorotannin Sargassum sp. extract on colon profile of diabetic rats. IOP Conf Ser Earth Environ Sci. 2020;493(1):1–8.
Karthick V, Akhila C, Kumar VG, Subashini D, Dhas TS, Govindaraju K, et al. In vitro anticancer activity of Sargassum sp. Polysaccharides against MCF-7 cell lines. Indian J Geo-Marine Sci. 2019;48(8):1267–73.
Hervidea R, Widiastuti EL, Nurcahyani E, Sutyarso, Susanto GN. Efek Ekstrak Metanol Makroalga Cokelat (Sargassum sp.), Merah (Gracillaria sp.) dan Taurin Terhadap Gambaran Histopatologi Hepar Mencit Jantan (Mus musculus) yang Diinduksi Benzo(α)Piren. J Biol Indones. 2018;14(1):123–31.
Fitri E. Respon Stres Pada Pasien Kritis. J Keperawatan Sriwij. 2014;1(1):86–93.
Kantasa VD, Kusumawardani B, Herniyati. Efek Stresor Rasa Sakit Renjatan Listrik terhadap Limfosit dan Makrofag pada Gingiva Tikus Sprague Dawley ( The Effect of Electrical Shock Stressor on Lymphocytes and Macrophages in Gingival Tissue of Sprague Dawley Rats). Pustaka Kesehtan. 2016;4(1):48–54.
Ronchetti S, Ricci E, Migliorati G, Gentili M, Riccardi C. How glucocorticoids affect the neutrophil life. Int J Mol Sci. 2018;19(12):1–12.
Ivetic A, Louise H, Green H, Hart SJ. L-selectin: A Major Regulator of Leukocyte Adhesion , Migration and Signaling. Front Immunol. 2019;10(1):1–22.
Herlambang, Fitri AD, Djauhari HA. Buku pedoman penulisan skripsi. Djauhari HA, Lina, editors. Jambi: Fakultas Kedokteran dan Ilmu Kesehatan Universitas Jambi; 2017. 17 p.
Na’mah NU. Pengaruh murottal al-quran terhadap kadar leukosit mencit (mus musculus) jantan yang mengalami stres. Universitas Islam Negeri Maulana Malik Ibrahim; 2020.
Gazali M, Nurjanah N, Zamani NP. Eksplorasi Senyawa Bioaktif Alga Cokelat Sargassum sp. Agardh sebagai Antioksidan dari Pesisir Barat Aceh. J Pengolah Has Perikan Indones. 2018;21(1):167–78.
Naina Y, Wulandari R, Said T, Yuni N, Rika W, Tengku RIS. Skrining Komponen Bioaktif Ethanol 96 % Sargassum sp . sebagai Antibakteri Terhadap Vibrio Harveyi. Intek Akuakultur. 2019;3(2):22–33.
Ardina R, Rosalinda S. Morfologi eosinofil pada apusan darah tepi menggunakan pewarnaan giemsa, wright, dan kombinasi wright-giemsa. J Surya Med. 2018;3(2):5–12.
Fitri ZE. Klasifikasi trombosit pada citra hapusan darah tepi berdasarkan gray level co-occurrence matrix menggunakan backpropagation. Institut Teknologi Sepuluh Nopember Surabaya; 2017.
Bakhri S. Analisis Jumlah Leukosit Dan Jenis Leukosit Pada Individu Yang Tidur Dengan Lampu Menyala Dan Yang Dipadamkan. J Media Anal Kesehat. 2018;1(1):83–91.
Berawi KN, Agverianti T. Efek Aktivitas Fisik pada Proses Pembentukan Radikal Bebas sebagai Faktor Risiko Aterosklerosis. J Major [Internet]. 2017;6(2):86–91. Available from: http://juke.kedokteran.unila.ac.id/index.php/majority/article/view/1019
Tarigan AP, Harahap NS, Marpaung DR. Pengaruh Pemberian Jus Buah Naga Merah Setelah Latihan Fisik Intensitas Berat Terhadap Jumlah Leukosit. J Keolahragaan. 2020;8(2):140–7.
Wahid S, Miskad UA. Imunologi lebih mudah dipahami. 1st ed. Wijaya A, editor. Makassar: Brilian Internasional Surabaya; 2016. 13–17 p.
Yudhawati R, Prasetiyo YD. Imunopatogenesis Penyakit Paru Obstruktif Kronik. J Respirasi. 2019;4(1):19–25.
Hendrajid Z, Taihuttu YMJ, Laura BS, Latuconsina VZ. Jenis leukosit mencit ( mus musculus ) pasca stres akut dengan perlakuan ekstrak etanol biji pala ( myristica fragrans houtt ). Pattimura Med Rev. 2020;2(2):103–16.
Wohleb ES, Mckim DB, Sheridan JF, Jonathan P, Anisman H. Monocyte trafficking to the brain with stress and inflammation: a novel axis of immune-to-brain communication that influences mood and behavior. Front Neurosci. 2015;8(January):1–17.
Amri F. Farmakologi alprazolam dalam mengatasi gangguan panik. J Kedokt Syiah Kuala. 2012;12(3):187–90.
Hardiningtyas SD, Purwaningsih S, Handharyani E. Antioxidant activity and hepatoprotective effect of green mangrove leaves. JPHPI. 2014;17(1):80–91.
Parwata IMO. Flavonoid [Internet]. Universitas Udayana; 2016. Available from: https://simdos.unud.ac.id/uploads/file_pendidikan_1_dir/c0c585d54a388056ea08899533164330.pdf
Suhendra CP, Widarta IWR, Wiadnyani S. Pengaruh konsentrasi etanol terhadap aktivitas antioksidan ekstrak rimpang ilalang (imperata cylindrica ( l ) beauv .) Pada ekstraksi menggunakan gelombang. J Ilmu dan Teknol Pangan. 2019;8(1):27–35.
Noviyanti. Antioksidan ekstrak etanol daun jambu brazil batu ( psidium guineense l .) Dengan metode dpph. J Farm Bahari. 2016;7(1):29–35.
Herold MJ, Mcpherson KG, Reichardt HM. Glucocorticoids in T cell apoptosis and function. Cell Mol Life Sci. 2006;63(1):60–72.
Collins KG, Fitzgerald GF, Stanton C, Ross RP. Looking Beyond the Terrestrial: The Potential of Seaweed Derived Bioactives to Treat Non-Communicable Diseases. Mar Drugs. 2016;14(60):1–31.
Frankiensztajn LM, Elliott E, Koren O. ScienceDirect The microbiota and the hypothalamus-pituitary-adrenocortical ( HPA ) axis , implications for anxiety and stress disorders. Curr Opin Neurobiol [Internet]. 2020;62(1):76–82. Available from: https://doi.org/10.1016/j.conb.2019.12.003
Puspitasari L. Ekstrak etanol daun pandan wangi (pandanus amaryllifolius r.) 10% menurunkan immobility time dan kadar kortisol tikus jantan galur wistar yang depresi. Intisari Sains Medis. 2017;8(1):24–30.
Adrielly C, Ferraz A, Gonçalves R, Júnior DO, Picot L, Roberto J, et al. Pre-clinical investigations of β-carboline alkaloids as antidepressant agents: A systematic review. Fitoterapia [Internet]. 2019;137(1):1–14. Available from: https://doi.org/10.1016/j.fitote.2019.104196
Primasari N. Gambaran morfologi sel eosinofil dan limfosit pada sediaan apusan darah tipis dalam pewarnaan giemsa yang diencerkan menggunakan nacl fisiologis dan aquadest. Politeknik Kesehatan Kendari; 2018.
Aulia R, Hasan M, Karmil TF. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract. J Med Vet. 2017;11(2):93–9.
Karasuyama H, Miyake K, Yoshikawa S, Yamanishi Y. Multifaceted roles of basophils in health and disease. J Allergy Clin Immunol [Internet]. 2018;142(2):370–80. Available from: https://doi.org/10.1016/j.jaci.2017.10.042
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