Larvicidal activity of Aloe vera leaf extract against Aedes aegypti

Keywords: Aloe vera, Aedes aegypti, larvicide, mortality, fourth instar larvae.

Abstract

Aedes aegypti is the main vector of various arbovirus diseases, so effective and natural-based control alternatives are needed. Aloe vera is known to contain various bioactive compounds that have the potential to have insecticidal activity. This study aims to evaluate the effectiveness of Aloe vera leaf extract as a larvicide against fourth-instar A. aegypti. The study used fourth-instar A. aegypti larvae with A. vera extract concentrations of 0%, 0.3%, 0.6%, 0.9%, and 1.1%. Each treatment used 25 larvae with three replications. Larval mortality was observed after 24 hours. Data were analyzed using one-way ANOVA, Tukey's follow-up test, and probit analysis. A. vera extract showed increasing larvicidal activity with increasing concentration. The difference in mortality between treatments was statistically significant (p<0.001), and all concentration groups showed significant differences from each other based on Tukey's test. The LC50 value was found at a concentration of 0.591%. A. vera leaf extract has the potential as a botanical larvicide against fourth instar A. aegypti.

Downloads

Download data is not yet available.

References

Anindita, R., Mayasari, E., Prastiwi, A. D., Alamsyah, R. A., & Inggraini, M. (2022). Bioactivity Test of Bitter Melon (Momordica charantia L.) Ethanol Extract As Larvacide on Aedes sp and Culex sp. Indonesian Journal of Biology Education, 5(1), 14–21. https://doi.org/10.31002/ijobe.v5i1.6028

Arianto, B., & Aditama, W. (2024). Toxicity Test of Difference in Concentration of Aloe Vera (Aloe vera) as a Larvicide of Aedes aegypti. ASJo: Aceh Sanitation Journal, 3(2), 165–177. https://doi.org/10.31101/ijhst.v7i2.4308

Aryakia, E. (2026). Uncovering the Key Factors Influencing Phytochemical and Phytopharmacological Properties During Medicinal Plant Processing. EFood, 7(1), 1–65. https://doi.org/10.1002/efd2.70105

Chore, J. K., Obonyo, M., Wachira, F. N., & Mireji, P. O. (2014). Larvicidal activity of selected aloe species against aedes aegypti (Diptera: Culiciade). Journal of Insect Science, 14(1), 1–3. https://doi.org/10.1093/jisesa/ieu064

De Souza Wuillda, A. C. J., Martins, R. C. C., & Costa, F. D. N. (2019). Larvicidal activity of secondary plant metabolites in aedes aegypti control: An overview of the previous 6 years. In Natural Product Communications (Vol. 14, Number 7). SAGE Publications Inc. https://doi.org/10.1177/1934578X19862893

Demarque, D. P., & Espindola, L. S. (2021). Challenges, Advances and Opportunities in Exploring Natural Products to Control Arboviral Disease Vectors. Frontiers in Chemistry, 9, 1–10. https://doi.org/10.3389/fchem.2021.779049

Engdahl, C. S., Tikhe, C. V., & Dimopoulos, G. (2022). Discovery of novel natural products for mosquito control. Parasites and Vectors, 15(1). https://doi.org/10.1186/s13071-022-05594-z

Inaba, K., Ebihara, K., Senda, M., Yoshino, R., Sakuma, C., Koiwai, K., Takaya, D., Watanabe, C., Watanabe, A., Kawashima, Y., Fukuzawa, K., Imamura, R., Kojima, H., Okabe, T., Uemura, N., Kasai, S., Kanuka, H., Nishimura, T., Watanabe, K.,Niwa, R. (2022). Molecular action of larvicidal flavonoids on ecdysteroidogenic glutathione S-transferase Noppera-bo in Aedes aegypti. BMC Biology, 20(1), 1–20. https://doi.org/10.1186/s12915-022-01233-2

Kumar, S., Yadav, M., Yadav, A., Rohilla, P., & Yadav, J. P. (2017). Antiplasmodial potential and quantification of aloin and aloe-emodin in Aloe vera collected from different climatic regions of India. BMC Complementary and Alternative Medicine, 17(1), 1–10. https://doi.org/10.1186/s12906-017-1883-0

Lubis, R., Ilyas, S., & Panggabean, M. (2018a). The effectivity test of Aloe vera leaf extract to larvae AEDES sp. Asian Journal of Pharmaceutical and Clinical Research, 11(7), 262–266. https://doi.org/10.22159/ajpcr.2018.v11i7.24463

Lubis, R., Ilyas, S., & Panggabean, M. (2018b). The effectivity test of Aloe vera leaf extract to larvae AEDES sp. Asian Journal of Pharmaceutical and Clinical Research, 11(7), 262–266. https://doi.org/10.22159/ajpcr.2018.v11i7.24463

Nurul ’Ain, A. D., & Pratiwi, R. D. (2025). Mapping the spread of dengue fever with geographic information system in Magelang City in 2020-2024. International Journal of Health Science and Technology, 7(2), 165–177. https://doi.org/10.31101/ijhst.v7i2.4308

Oxborough, R. M., Emidi, B., Yougang, A. P., Abeku, T. A., Ahmed, F., Biggs, J. R., Chan, K., Cook, J., Edwards, A., Falconer, J., Kamgang, B., Messenger, L. A., Seelig, F., Taylor, R., Tedjou, A. N., Lines, J., Clarke, S. E., & Kristan, M. (2025). Building resilience against the growing threat of arboviruses: a scoping review of Aedes vector surveillance, control strategies and insecticide resistance in Africa. Parasites and Vectors , 18(1). https://doi.org/10.1186/s13071-025-07049-7

Pelah, D., Abramovich, Z., Markus, A., & Wiesman, Z. (2002). The use of commercial saponin from Quillaja saponaria bark as a natural larvicidal agent against Aedes aegypti and Culex pipiens. Journal of Ethnopharmacology, 81, 407–409. www.elsevier.com/locate/jethpharm

Pourzangiabadi, M., Najafi, H., Fallah, A., Goudarzi, A., & Pouladi, I. (2025). Dengue virus: Etiology, epidemiology, pathobiology, and developments in diagnosis and control – A comprehensive review. Infection, Genetics and Evolution, 127(2025), 1–14. https://doi.org/10.1016/j.meegid.2024.105710

Rahmayani, S., Satoto, T. B. T., & Mahardika. (2019). Aktivitas Larvisida Kulit Lidah Buaya (Aloe Vera) Terhadap Larva Nyamuk Aedes aegypti (Diptera: Culicidae) [Gadjah Mada University]. https://doi.org/http://etd.repository.ugm.ac.id/

Rakhmani, A. N., & Zuhriyah, L. (2024). Knowledge, Attitudes, and Practices Regarding Dengue Prevention Among Health Volunteers in an Urban Area – Malang, Indonesia. Journal of Preventive Medicine and Public Health, 57(2), 176–184. https://doi.org/10.3961/jpmph.23.484

Sánchez, M., González-Burgos, E., Iglesias, I., & Gómez-Serranillos, M. P. (2020). Pharmacological update properties of aloe vera and its major active constituents. Molecules, 25(6), 1–37. https://doi.org/10.3390/molecules25061324

Santos, E. F., Anselmo, W. M., de Lemos, E. E. P., de Oliveira Farias de Aguiar, J. C. R., da Silva, A. C., Santos, F. H. G. dos, Arruda, C. C. L., Aguiar, J. V. C., de Andrade, J. J. A., da Rocha, S. K. L., Araújo, L. de A., Pereira Júnior, P. G., Albuquerque, C. F. de O., Sousa, E. da S., dos Santos, G. L., da Conceição, T. Z., de Andrade, L. A., Soares, L. A. L., Ferreira, M. R. A., & Navarro, D. M. do A. F. (2025). Larvicidal Activity of Essential Oil, Hydrolate, and Aqueous Extract from Leaves of Myrciaria floribunda Against Aedes Aegypti. Molecules, 30(15), 1–14. https://doi.org/10.3390/molecules30153116

Sieumeni, A., Oumarou, K. M., Younoussa, L., Moutsina, K. K., Kowa, T. K., & Nukenine, E. N. (2026a). Toxicity of the Methanol Extract and the Fractions of the Leaves of Lippia adoensis Hochst (Verbenaceae) against the Larvae of Anopheles gambiae Giles and Culex quinquefasciatus Say (Diptera: Culicidae). Advances in Entomology, 14(03), 234–249. https://doi.org/10.4236/ae.2026.143014

Sieumeni, A., Oumarou, K. M., Younoussa, L., Moutsina, K. K., Kowa, T. K., & Nukenine, E. N. (2026b). Toxicity of the Methanol Extract and the Fractions of the Leaves of Lippia adoensis Hochst (Verbenaceae) against the Larvae of Anopheles gambiae Giles and Culex quinquefasciatus Say (Diptera: Culicidae). Advances in Entomology, 14(03), 234–249. https://doi.org/10.4236/ae.2026.143014

Silva, R. M. da, Neves, J. H., Wolf, P. F., Wolf, A. C. M., Soper, M. S., Bassuino, M. S., Moyses, F. dos S., Pereira, V. R. Z. B., Borges, G. R., Kist, L. F., Wolf, L. M., & Wolf, J. M. (2026). Dengue Virus in the 21st Century: Transmission, Pathogenesis, and Climate-Driven Challenges. Zoonotic Diseases, 6(3), 1–20. https://doi.org/10.3390/zoonoticdis6030033

Silva, R. L., Demarque, D. P., Dusi, R. G., Sousa, J. P. B., Albernaz, L. C., & Espindola, L. S. (2020). Residual Larvicidal Activity of Quinones against Aedes aegypti. Molecules, 25(17), 1–12. https://doi.org/10.3390/molecules25173978

Subramaniam, J., Kovendan, K., Mahesh Kumar, P., Murugan, K., & Walton, W. (2012). Mosquito larvicidal activity of Aloe vera (Family: Liliaceae) leaf extract and Bacillus sphaericus, against Chikungunya vector, Aedes aegypti. Saudi Journal of Biological Sciences, 19(4), 503–509. https://doi.org/10.1016/j.sjbs.2012.07.003

Weeratunga, P., Rodrigo, C., Fernando, S. D., & Rajapakse, S. (2017). Control methods for Aedes albopictus and Aedes aegypti. Cochrane Database of Systematic Reviews, 2017(8). https://doi.org/10.1002/14651858.CD012759

World Health Organization (WHO). (2005). Guidelines for laboratory and field testing of mosquito larvicides. World health organization communicable disease control, prevention and eradication who pesticide evaluation scheme. https://doi.org/WHO/CDS/GCDPP.15p.

World Health Organization (WHO). (2025). Biweekly Epidemiological Bulletin Who Health Emergencies Programme Who Regional Office For South-East Asia. https://doi.org/althbulletinoncurrentDenguesituationpublishedbyDGHS.(2024).Availableat:https://old.dghs.gov.bd/index.php/bd/home/5200-daily-dengue-status-report

Published
2026-09-24
How to Cite
Anindita, R., & Izharoh, I. (2026). Larvicidal activity of Aloe vera leaf extract against Aedes aegypti. RUMPHIUS Pattimura Biological Journal, 8(2), 125-132. https://doi.org/10.30598/rumphiusv8i2p125-132