Uji Fitokimia dan Toksisitas Ekstrak Etil Asetat Kulit Batang Melochia umbellata (Houtt) Stapf. var. Visenia dengan Metode Brine Shirmp Lethality Test (BSLT)

Phytochemical And Toxicity Assay Extract Ethyl Acetate of Melochia umbellata (Houtt) Stapf. var. Visenia By Brine Shirmp Lethality Test Method

  • Fauziah Ahmad Department of Chemistry, Faculty of Mathematics and Natural Science, University of Hassanuddin-Indonesia
  • Nunuk H. Soekamto Department of Chemistry, Faculty of Mathematics and Natural Science, University of Hassanuddin-Indonesia
  • Firdaus Firdaus Department of Chemistry, Faculty of Mathematics and Natural Science, University of Hassanuddin-Indonesia
Keywords: Melochia umbellata (Houtt) Stapf. var. Visenia, alkaloid, terpenoid, steroid, Artemia salina Leach

Abstract

Melochia umbellata (Houtt) Stapf. var. Visenia is plant species included in Malvaceae family. This spesies is known as paliasa and used as traditional medicine by the people of South Sulawesi. This study aimed to determine the secondary metabolites by reagents and toxicity characteristic testing from bark extract of Melochia umbellata (Houtt) Stapf. var. Visenia using Brine Shirmp Lethality Test (BSLT) method. The extract was prepeared by maceration ethyl acetate. Phytochemical test result showed that the ethyl acetate extract was containing the steroid compound, alkaloids and terpenoids. Toxicity test performed by shrimp Artemia salina Leach larvae was at 48 hours. The toxic effects of the extract were identified by the percentage of the number of shrimp larvae mortality using probit value analysis (LC50). The toxicity test of bark Melochia umbellata (Houtt) Stapf. var. Visenia extract showed that the ethyl acetate extract is toxic to A. salina  LC50 = 54,55 mg / mL.

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Published
2017-01-31
How to Cite
(1)
Ahmad, F.; Soekamto, N.; Firdaus, F. Uji Fitokimia Dan Toksisitas Ekstrak Etil Asetat Kulit Batang Melochia Umbellata (Houtt) Stapf. Var. Visenia Dengan Metode Brine Shirmp Lethality Test (BSLT). Indo. J. Chem. Res. 2017, 4, 378-381.
Section
Artikel Penelitian (Research articles)

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