THE EFFECT OF SEVERAL TOOTHPAST ON THE GROWTH OF Streptococcus mutans
Abstract
This study aims to determine the effect of several toothpastes on the growth of Streptococcus mutans which is the main cause of tooth decay. The study used 5 types of toothpaste, namely toothpaste A, toothpaste B, toothpaste C, toothpaste D, and toothpaste E. The antibacterial testing method used the diffusion method with the cup-plate technique. The significance level of each type of toothpaste was measured using a completely randomized design followed by a Tukey test to see differences in toothpaste treatment. The results showed that toothpaste A with active ingredients Monofluorophosphate Sodium 1.12%, Triclosan 0.3%, Calcium Glycerophosphate 0.13% had the largest average resistance zone of 27.3 mm. Whereas Toothpaste E with the active ingredient 0.08% Sodium Monofluorophosphate, 0.01% Sodium Fluoride has the smallest average resistance zone, which is 14.25 mm. The diameter of the ANOVA test zone of several types of barrier toothpaste against Streptococcus mutans at 95% confidence intervals showed that there was a significant difference.
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References
Astuti, Purbosari Y. 2008. Analysis of the Influence of Brand Equity on the Formation of Customer Loyalty in Types of Toothpaste Brands Using Sem Analysis (Structural Equation Modeling).
Aguilar F, Charrondiere UR, Dusemund B, Galtier P, Gilbert J, Gott DM. Sodium Monofluorophosphate as a source of fluoride added for nutritional purposes to food supplements. EFSAJ 2008; 886: 1 - 18.
Ferdinand, F and Ariwibowo, M. 2007. Practical Learning of Biology. Jakarta: Visindo Media Persada
Gerald, I. Roth. D.D.S. & Roberts, Calmes. 1998. Basic Microbiology in Practice. Jakarta: Gramedia Pustaka Utama
Jawetz E, Melnick, Adelberg. 2001. Medical Microbiology, Ed. 22nd, McGraw Hill Companies USA: 229 – 31.
Sharma A and Somani R. 2018. Dermatoglyphic Interpretation of Dental Caries and Its Correlation to Salivary Bacteria Interactions: An in vivo Study. J Ind Soc Ped Prev Dent 27: 17 – 21.
Nick, J. 2009. Ingredients of Toothpaste. http://www.buzzle.com/articles/ingredients-intoothpaste.html. Accessed on January 25 2020, at 01:43 WIT.
Kidd, E. A. M., and Bechal, S. J. 1992. Basics of Caries: Disease and Management. Ed. 2nd. Jakarta: EGC Medical Book Publishers.
Hadioetomo, Ratna S. 1990. Basic Microbiology in Practice. Basic Laboratory Techniques and Procedures. Jakarta: PT. Scholastic.
Taihuttu, Y.M.J. 2007. Effect of the Inhibitory Power of Areca Seed Extract (Areca catechu L.) on the growth of the bacteria Streptococcus mutans in vitro. Unpublished Thesis. Faculty of Mathematics and Natural Sciences Unpatti Ambon.
Pratiwi, Rini. 2005 Differences in the Inhibitory Power Against Streptococcus mutans of Several Toothpastes Containing Herbals. (http://ojs.lib.unair.ac.id/index.php/dj/article/viewFile930/927). Accessed on September 8 2020, at 01:05 WIT.
Pratama, Moch Rachdie. 2005. The Effect of Miswak (Salvadora persica) Wood Powder Extract on the Growth of Streptococcus mutans and Staphylococcus aureus Bacteria Using the Agar Diffusion Method. http://thesis.blogsome.com/. Accessed on September 27 2019, at 01:05 WIT.
Hanafiah, Ali Kemas. 2002. Experimental Design Theory and Applications, Third Edition. Jakarta: PT Grafindo Persada.
Restina, navella. 2008. Effect of Toothpaste Containing Xylitol and Sodium Monofluorophosphate on the Growth of Streptococcus sp. On Dental Plaque.
http://urseek40.vmn.net/search.php?lg=en&type=dns&tbn=oovoo2_0dn&q=http://digilib.unej.ac.id/ go.php. Accessed on January 6 2018, at 08:32 WIT.
Ministry of Health. Guidelines for the use of fluorides. New Zealand: New Zealand Guideline Groups; 2009.
Klimek J, Jung M, Jung S. 1997. Interindividual Differences In Degradation Of Sodium Monofluorophosphate By Saliva In Relation To Oral Health Status. Dental Clinic, Justus-Liebig University, Giessen, Germany. Arch Oral Biol. 1997 Feb; 42(2):181 – 4.
Jackson, L. R. 1982. In vitro Hydrolysis of Monofluorophosphate by Dental Plaque Microorganisms. J Dent Res 61(7): 953 – 956,
Van Loveren, C., J. F. Buijs, Ten. 2000. The Effect Of Triclosan Toothpaste On Enamel
Demineralization In A Bacterial Demineralization Model. J. Antimicrobial. Chemother. 45(2): 153 – 158.
Vyas, Yoger Kumar, Maheep Bhatnagar, Khanika Sharma. 2008. In vitro evaluation of Antibacterial Activity of An Herbal Dentrifrice Against Streptococcus mutans and Lactobacillus acidophilus. India J Deny Res, 19 (1).
Jannesson, L., S. Renvert, P. Kjellsdotter, A. Gaffar, N. Nabi, D. Birkhed. 2002. Effect of A Triclosan Containing Toothpaste Supplemented with 10% Xylitol on Mutans Streptococci in Saliva and Dental Plaque A 6-Month Clinical Study. Caries Res 36:36-39
Imasatto S, Torii M, Tsuchitani Y. 1995. Antibacterial effect of composite incorporating Triclosan against Streptococcus mutans. J Osaka Univ Dent Sch. 1995 Dec;35:5 – 11.
Phan, Tuan-Nghia and Robert E. Marquis. 2006. Triclosan Inhibition Of Membrane Enzymes And Glycolysis Of Streptococcus mutans In Suspensions And Biofilms. Can. J. Microbiol. 52(10): 977 – 983.
Lynch, R. J. M. 2004. Calcium Glycerophosphate And Caries: A Review Of The Literature. International Dental Journal (2004) 54, 310 – 314.
Lynch, R. J. M. and Cate, J. M. ten. 2006. Effect of Calcium Glycerophosphate on Demineralization in an in vitro Biofilm Model. Caries Res 2006;40:142 – 147
Tenuta, L. M. A., Cenci, T., Pereira, C. P. M., Tabchoury, A. A., Del Bel Cury, and J. A. Cury. 2006. Calcium Glycerophosphate Fluoride Dentifrice Effect on Enamel Demineralization In situ. Faculty of Dentistry of Piracicaba, Brazil.
Gonçalves, N. C. L. A. de. V., Valsecki Junior, A., Salvador, S. L. de. S., Bergamo, G. C. 2006. Effect Of Mouth Rinses Containing Xylitol And Sorbitol On The Number Of Streptococcus mutans In The Human Mouth. Pan American Journal of Public Health.
Caufield, Page W and Yvonne M. Wannemuehler. 1982. In vitro Susceptibility of Streptococcus mutans 6715 to Iodine and Sodium Fluoride, Singly and in Combination, at Various pH Values. Antimicrobial Agents And Chemotherapy 22(1):115-119.
Lobo, P. L. D., C. B. M. de Carvalho, S. G. C. Fonseca, R. S. L. de Castro, A. J. Monteiro, M. C.
Fonteles, C. S. R. Fonteles. 2008. Sodium Fluoride And Chlorohexidine Effect In The Inhibition Of Mutans Streptococci In Children With Dental Caries: A Randomized, Double-Blind Clinical Trial. Oral Microbiology and Immunology 23(6): 486 – 491.
Sari, Kiki Mia Kumala. 2008. Effect of Toothpaste Containing Cetylpyridinium Chloride and Sodium Fluoride on the Growth of Streptococcus sp. On Dental Plaque. Gray literature from GDLHUB / 2008-03-27 14:57:02. http://digilib.unej.ac.id/go.php?id=gdlhub-gdl-grey-2008kikimiakum-1211&PHPSESSID=jgzmgejbfvbfhwmi. Accessed on January 5 2018, at 05:05 WIT.
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