ANTIBACTERIAL ACTIVITY OF BINAHONG ROOT EXTRACT (Anredera cordifolia (Ten.) Steenis) IN TREATMENT OF BOILS
Abstract
Infectious diseases caused by Staphylococcus aureus can be cured with traditional medicine, One example of a plant that has antibacterial power is binahong root. This study aims to determine the minimum inhibitory concentration (MIC) and minimum killing concentration (MBC) of binahong root extract against the growth of Staphylococcus aureus bacteria. Binahong root extract was obtained from maceration using absolute ethanol solvent. Furthermore, the extract obtained was made with a concentration dilution of 5%, 10%, 15% and 20%. The method used is disc diffusion. The results showed that each concentration of binahong root extract had an inhibitory effect on the growth of Staphylococcus aureus bacteria. This is influenced by the active compounds contained in the binahong root extract such as flavonoids, saponins, tannins, and alkaloids. Binahong root extract concentrations of 5%, 10%, 15%, and 20% were found to have zones of inhibition against the growth of S. aureus bacteria and the minimum inhibitory level (MIC) of Binahong root extract against the growth of S. aureus bacteria at a concentration of 5%.
Downloads
References
Agus, R. M., Enny, F., & Dewi, K. 2013. Isolation, Identification and Testing of the Antibacterial Activity of Triterpenoid Compounds from Binahong (Anredera cordifolia (Ten.) Steenis) Leaf Extract against Staphylococcus aureus and Escherichia coli. Chem Info Journal. Vol 1 (1). 328 – 336.
Ani, U., Dwi, K., Diah, TM. 2012. Effect of Giving Binahong (Anredera cordifolia (Ten) Steenis) Leaf Extract on the Healing of Staphylococcus aureus Infected Wounds in Mice. Science Health Analysis. 01 (2) : 68-75.
Anggun, A. W. 2012. Test of the Antibacterial Activity of Ethanol Extract of Binahong Leaves (Anredera cordifolia (Ten.) Steenis) Against S. aureus Bacteria In Vitro, Thesis. Medical School. University of Jember.
Danan, Habibah SS, Rahmawati I. 2019. The influence of binahong leaves (solution Anredera cordofolia (Ten) Stennison gingivitic) healing in SMP 13 Cempaka Banjarbaru students. J Health Polytechnic Health Scale. Banjarmasin;10 (2):91-98.
Davis, W. W., & Stout T. R. 1971. Disc plate method of microbiological antibiotic assay. American Society for Microbiology, 4(22). p 666-670.
Fatisa Y. 2013. Antibacterial effect of peel and seed extracts of pulasan fruit (nephelium mutabile) against staphylococcus aureus and escherichia coli in vitro. Animal Husbandry Journal;10(1):31-38.
Fitriah, Mappiratu, Prismawiryanti. 2017. Test of the antibacterial activity of Johar plant leaf extract (Cassia siamea Lamk) from several levels of solvent polarity. Covalent. 3(3): 242-251.
Gibson, G.G., Skeet, P. 1991. Introduction to Drug Metabolism, translated by Iis Aisyah b., 1-30, Publisher, University of Indonesia, Jakarta.
Ginting P, Faisal H, Hanum SF, From RW. 2020. Activity gel of binahong leaf extract (Anredera cordifolia (ten.) steenis) againts cut wound healing infected with staphylococcus aureus bacteria in rabbits (oryctolagus cuniculus). Pharmaceutical World Journal; 4(3):116-125.
Jamilah, M. 2010. Comparison of the Effectiveness of Toothpaste Containing Betel Leaf Extract and Fluorine on the Growth of Streptococcus mutans (In Vitro). Thesis. Faculty of Dentistry. University of North Sumatra.
Kusuma. 2009. Staphylococcus aureus paper. Padjajaran University. Bandung.
Manik, D.F., Hertiani, T., and Anshory, H. 2014. Correlation analysis between flavonoid levels and the antibacterial activity of ethanol extract and cherry leaf fractions (Muntingia calaburam L.) against Staphylococcus aurues. Khazanah, 6(2):1-11.
Manoi F. 2009. Binahong (Anredera cordifolia) as medicine. Industrial Plant Research and Development Newsletter. 15 (1):p 3-5.
Mastuti, R. 2016. Secondary Metabolites and Plant Defense. 3rd Ed. Malang, Brawijaya University.
Mathers, C., T. Boerma & Fat D. M. 2008. The Global Burden of Disease 2004 Update. World Health Organization.
Nasronuddin. 2007. Infectious Diseases in Indonesia. Current and Future Solutions. Airlangga University Press: Surabaya.
Noorhamdani, A. S., Sudiarto, & Vita, U. 2010. Test of Binahong Leaf Extract as an Antimicrobial against Staphylococcus aureus and Pseudomonas aeruginosa. Thesis. Medical School. Brawijaya University. Poor.
Otieno, J.N., Kennedy M.M.H., Herbert V.L., & Rogasian L.A.M. 2008. Multi Plant or Single Plant Extracts, Which Is the Most Effective for Local Healing in Tanzania?. Afr. J. Trad. CAM. 5(2): 165-172.
Oxoid. 2006. Oxoid Manual. Edition 9. Oxoid Limited : Bandung.
Perwita, F.A. 2011. Purple Leaf Extract Technology in 70% Ethanol Using the Pecolation Method. Faperta. Sega Maret University, Serakarta.
Pratama, M. R. 2005. The effect of miswak (Salvador persica) wood powder extract on the growth of Streptococcus mutans and Staphylococcus aureus bacteria using the Agar Plate Diffusion Method. Thesis. Faculty of Mathematics and Natural Sciences. Biology Study Program. Sepuluh November Institute of Technology, Surabaya.
Prayudi Y. Warta Research Institute for Spices and Medicinal Plants. 2009.p.6.
Rachman A, Wardatun S, Wiendarlina IY. 2018. Isolation and Identification of Saponin Compounds from Methanol Extract of Binahong Leaves (Anredera cordifolia (Ten.) Steenis). Online Journal of Pharmacy Students.; 1(1).
Ravi, L., Manasvi, V., and Praveena, L.B. 2016. Antibacterial and antioxidant activity of saponin from Abutilon indicum leaves. Asian Journal Pharm, 9:344-347.
Rimporok S, Kepel BJ, Siagian KV. 2015. Testing the effectiveness of binahong (anredera cordifolia steenis) leaf extract on the growth of streptococcus mutans in vitro. Unsrat;4(4):15-21
Saputra S. Effect of Binahong (Anredera Cordifolia Steenis) Leaf Extract on the Growth of Salmonella Typhi. Journal. 2008. p.1-2.
Sari, N.E. 2012. Test of the Inhibitory Power of Breadfruit Leaves (Artocarpus altilis folium) against Candida albicans fungi and Bacilus subtilis bacteria using the diffusion method. Scientific Writing, AKAFARMA Lampung.
Sudarmono, P., 1993, Textbook of medical microbiology: Genetics and Resistance, Literacy Development, University of Indonesia, Jakarta.
Sulistyani S. 2013. Testing the Effect of 70% Ethanol Extract of Binahong Leaves (Anredera cordifolia Steenis) on Alt (Alanine Aminotransferase) Levels in Male Wistar Rats (Rattus norvegicus) Induced with Paracetamol. Medical Journal. p.3.
Susanti, N. 2016. Antimicrobial activity of jeringau rhizome extract against the growth of Candida albicans. Biodjati Journal, 1(1):55-58.
Sulastrianah., Imran, and Fitria, E.S. 2014. Test of the inhibitory power of soursop leaf extract (Annona muricata L) and betel leaf (Piper betle L) on the growth of Escherichia coli bacteria. UHO Journal, 1(1):76-84.
Tille, P. M. 2017. Bailey & Scott's Diagnostic Microbiology. In Basic Medical Microbiology (fourteenth, p. 45). St. Louis Missouri: Elsevier.
Wahyono, H. 2007. The role of clinical microbiology in treating infectious diseases. Inauguration Speech Paper for Professor in Microbiology, Faculty of Medicine, Diponegoro University
Ying L, Hernawan I. Inhibitory Power of Binahong Leaf Extract Against Polybacteria in Recurrent Aphthous Stomatitis (SAR). Oral Medicine Dental Journal. 2011;3(2):18-25.
Yuliana, S.R.I., Leman, M.A., and Anindita, P.S. 2015. Test of the inhibitory power of banana stem saponin compounds (Musa paradisiacal) on the growth of Candida albicans. e-GiGi Journal, 3(2):2.
Zimbro, M.J., D.A. Power, S.M. Miller, G.E. Wilson, and J.A. Johnson. 2009. Difco and BBL Manual, Manual of Microbiological Culture Media. Second Edition. Becton, Dickinson and Company. Maryland. America.
Authors who propose a manuscript and have it approved for publication know that the manuscript will be registered and become part of the RPBJ. Authors and readers understand that this journal is open and all its contents can be accessed freely, provided that RPBJ is still listed as the source of information. The hope is that this journal can become a vehicle for exchange and scientific knowledge for society and the scientific community, especially in the field of Biology and other branches of science.